10.1371/journal.pone.0074641.g003
Christina Greer
Christina
Greer
Moonsook Lee
Moonsook
Lee
Maaike Westerhof
Maaike
Westerhof
Brandon Milholland
Brandon
Milholland
Rebecca Spokony
Rebecca
Spokony
Jan Vijg
Jan
Vijg
Julie Secombe
Julie
Secombe
Overexpression of Myc increases mutation frequency and decreases lifespan at 29
Public Library of Science
2013
myc
mutation
decreases
lifespan
2013-09-06 01:45:58
Figure
https://plos.figshare.com/articles/figure/_Overexpression_of_Myc_increases_mutation_frequency_and_decreases_lifespan_at_29_/791152
<p>°<b>C.</b> (A) Western blot from five adult heads at 29°C for three days from <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as Act<sup>TS</sup>>+) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as Act<sup>TS</sup>>Myc). Myc is induced ∼9-fold in this system (LiCOR quantitation). (B) <i>lacZ</i> mutation frequency of <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 29°C for 5 days before <i>lacZ</i> analyses to allow transgene expression. Black solid areas indicate frequency of point mutations and white areas indicate frequency of genome rearrangements. *indicates statistical significance of p<0.01 (student’s t-test). (C) Lifespan analyses of females of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test).</p>