10.1371/journal.pone.0074641.g003 Christina Greer Christina Greer Moonsook Lee Moonsook Lee Maaike Westerhof Maaike Westerhof Brandon Milholland Brandon Milholland Rebecca Spokony Rebecca Spokony Jan Vijg Jan Vijg Julie Secombe Julie Secombe Overexpression of Myc increases mutation frequency and decreases lifespan at 29 Public Library of Science 2013 myc mutation decreases lifespan 2013-09-06 01:45:58 Figure https://plos.figshare.com/articles/figure/_Overexpression_of_Myc_increases_mutation_frequency_and_decreases_lifespan_at_29_/791152 <p>°<b>C.</b> (A) Western blot from five adult heads at 29°C for three days from <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as Act<sup>TS</sup>>+) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as Act<sup>TS</sup>>Myc). Myc is induced ∼9-fold in this system (LiCOR quantitation). (B) <i>lacZ</i> mutation frequency of <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). Adults were placed at 29°C for 5 days before <i>lacZ</i> analyses to allow transgene expression. Black solid areas indicate frequency of point mutations and white areas indicate frequency of genome rearrangements. *indicates statistical significance of p<0.01 (student’s t-test). (C) Lifespan analyses of females of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/+ (shown as “Act<sup>TS</sup>>+”) and of the genotype <i>lacZ</i> #2/Tub-Gal80<sup>TS</sup>; Actin-Gal4/UAS-Myc (shown as “Act<sup>TS</sup>>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test).</p>