Influence of Manumycin A on protein and mRNA expression levels of IL-17/IL-22/IL-23, GM-CSF, and IFN-γ. Savvas Petanidis Doxakis Anestakis Maria Argyraki Margarita Hadzopoulou-Cladaras Athanasios Salifoglou 10.1371/journal.pone.0073616.g003 https://plos.figshare.com/articles/figure/_Influence_of_Manumycin_A_on_protein_and_mRNA_expression_levels_of_IL_17_IL_22_IL_23_GM_CSF_and_IFN_947_/791147 <p>Effect of Manumycin A treatment (10 µM) on the (<b>A</b>) mRNA expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by RT-PCR analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before RT-PCR. 5 µg of total RNA was isolated from treated colon cells. Values represent the mean ± SD of three independent experiments. (<b>B</b>) protein expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by Western Blot analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before protein quantification. Values represent the mean ± SD of three independent experiments.</p> 2013-09-06 01:50:01 manumycin mrna