Influence of Manumycin A on protein and mRNA expression levels of IL-17/IL-22/IL-23, GM-CSF, and IFN-γ.
Savvas Petanidis
Doxakis Anestakis
Maria Argyraki
Margarita Hadzopoulou-Cladaras
Athanasios Salifoglou
10.1371/journal.pone.0073616.g003
https://plos.figshare.com/articles/figure/_Influence_of_Manumycin_A_on_protein_and_mRNA_expression_levels_of_IL_17_IL_22_IL_23_GM_CSF_and_IFN_947_/791147
<p>Effect of Manumycin A treatment (10 µM) on the (<b>A</b>) mRNA expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by RT-PCR analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before RT-PCR. 5 µg of total RNA was isolated from treated colon cells. Values represent the mean ± SD of three independent experiments. (<b>B</b>) protein expression levels of IL-17, IL-22, IL-23, GM-CSF, and IFN-γ in K-ras positive colorectal cancer patients as performed by Western Blot analysis. Patient stage D colon cells were treated with Manumycin A (10µM) for 24 hr, before protein quantification. Values represent the mean ± SD of three independent experiments.</p>
2013-09-06 01:50:01
manumycin
mrna