The accumulations of H<sub>2</sub>O<sub>2</sub> induced by pathogens in WT<i>, atg5-1</i>, <i>rbohD</i> and <i>atg5-1</i>×<i>rbohD</i>. Junjian Dong Wenli Chen 10.1371/journal.pone.0073091.g008 https://plos.figshare.com/articles/figure/_The_accumulations_of_H_2_O_2_induced_by_pathogens_in_WT_atg5_1_rbohD_and_atg5_1_215_rbohD_/785641 <p>A. The plants (4 weeks old) were infiltrated with <i>Pst</i> DC3000 (AvrRps4) (OD<sub>600</sub> = 0.2) or MgCl<sub>2</sub> (control). DAB staining of leaves from WT, <i>atg5-1</i>, <i>rbohD</i> and <i>atg5-1 </i>× <i>rbohD</i> were taken after 24 hpi, respectively. Experiments were performed three times with similar results. B. Show are <i>Arabidopsis</i> leaves after infiltrating <i>Pst</i> DC3000 (AvrRps4) (OD<sub>600</sub> = 0.2) or 10 mM MgCl<sub>2</sub> (control) for 6 hpi. Then the changes of 525 nm peak values in fluorescence emission spectra were scanned for 120 min. Excitation wavelength: 488 nm; Excitation slit width: 10 nm; Emission slit width: 8.5 nm; Scanning speed: 200 nm/min; Scanning wavelength range: 510-550 nm.</p> 2013-08-30 02:15:17 accumulations induced pathogens