10.1371/journal.pone.0074602.g006 Chung-Hao Lu Chung-Hao Lu Robert Kuo-Kuang Lee Robert Kuo-Kuang Lee Yuh-Ming Hwu Yuh-Ming Hwu Ming-Huei Lin Ming-Huei Lin Ling-Yu Yeh Ling-Yu Yeh Ying-Jie Chen Ying-Jie Chen Shau-Ping Lin Shau-Ping Lin Sheng-Hsiang Li Sheng-Hsiang Li Effect of exogenous PLAU and SERPINE2 on matrix gene expression and the hyaluronan status of cumulus cells during IVM. Public Library of Science 2013 exogenous plau serpine2 matrix hyaluronan cumulus cells 2013-08-30 02:13:27 Figure https://plos.figshare.com/articles/figure/_Effect_of_exogenous_PLAU_and_SERPINE2_on_matrix_gene_expression_and_the_hyaluronan_status_of_cumulus_cells_during_IVM_/785609 <p><b>COCs were incubated with SERPINE2 (0.06 mg/ml) or PLAU (20 IU) in IVM medium for 3, 6, or 16 h.</b> After culturing, cumulus cells were collected for qRT-PCR analyses or COCs were transferred onto slides and fixed for hyaluronan evaluation. A, qRT-PCR revealed <i>Has2</i> (a), <i>Vcan</i> (b), <i>Tnfaip6</i>, and <i>Ptx3</i> mRNA levels in cumulus cells. Bars indicate means ± SD of three independent experiments each. <sup>†</sup><i>P</i><0.05, *<i>P</i><0.0001 compared with the group that COCs cultured for 3 h in IVM medium. B, After culturing for 6 h, the hyaluronan contents in untreated COCs (a and e) or COCs treated with PLAU (c and g) or SERPINE2 (d and h) were revealed by treatment with or without (staining control, b and f) HABP (green) as described in Materials and Methods. For contrast, the slides were counterstained with Hoechst 33258 (blue, a–d) or photographed under differential interference contrast microscopy (e–h). Scale bars, 100 µm.</p>