10.1371/journal.pone.0074602.g006
Chung-Hao Lu
Chung-Hao
Lu
Robert Kuo-Kuang Lee
Robert
Kuo-Kuang Lee
Yuh-Ming Hwu
Yuh-Ming
Hwu
Ming-Huei Lin
Ming-Huei
Lin
Ling-Yu Yeh
Ling-Yu
Yeh
Ying-Jie Chen
Ying-Jie
Chen
Shau-Ping Lin
Shau-Ping
Lin
Sheng-Hsiang Li
Sheng-Hsiang
Li
Effect of exogenous PLAU and SERPINE2 on matrix gene expression and the hyaluronan status of cumulus cells during IVM.
Public Library of Science
2013
exogenous
plau
serpine2
matrix
hyaluronan
cumulus
cells
2013-08-30 02:13:27
Figure
https://plos.figshare.com/articles/figure/_Effect_of_exogenous_PLAU_and_SERPINE2_on_matrix_gene_expression_and_the_hyaluronan_status_of_cumulus_cells_during_IVM_/785609
<p><b>COCs were incubated with SERPINE2 (0.06 mg/ml) or PLAU (20 IU) in IVM medium for 3, 6, or 16 h.</b> After culturing, cumulus cells were collected for qRT-PCR analyses or COCs were transferred onto slides and fixed for hyaluronan evaluation. A, qRT-PCR revealed <i>Has2</i> (a), <i>Vcan</i> (b), <i>Tnfaip6</i>, and <i>Ptx3</i> mRNA levels in cumulus cells. Bars indicate means ± SD of three independent experiments each. <sup>†</sup><i>P</i><0.05, *<i>P</i><0.0001 compared with the group that COCs cultured for 3 h in IVM medium. B, After culturing for 6 h, the hyaluronan contents in untreated COCs (a and e) or COCs treated with PLAU (c and g) or SERPINE2 (d and h) were revealed by treatment with or without (staining control, b and f) HABP (green) as described in Materials and Methods. For contrast, the slides were counterstained with Hoechst 33258 (blue, a–d) or photographed under differential interference contrast microscopy (e–h). Scale bars, 100 µm.</p>