10.1371/journal.pone.0072790.g007 Guihua Liu Guihua Liu Xiangzhou Sun Xiangzhou Sun Jun Bian Jun Bian Rongpei Wu Rongpei Wu Xuan Guan Xuan Guan Bin Ouyang Bin Ouyang Yanping Huang Yanping Huang Haipeng Xiao Haipeng Xiao Daosheng Luo Daosheng Luo Anthony Atala Anthony Atala Yuanyuan Zhang Yuanyuan Zhang Chunhua Deng Chunhua Deng ADSCs-VEGF increased cavernous smooth muscle content in STZ-induced diabetic rats. Public Library of Science 2013 Computational biology Molecular genetics gene expression developmental biology stem cells mesenchymal stem cells genetics Model organisms Animal models rat Molecular cell biology Cellular types Clinical research design Animal models of disease Endocrinology Diabetic endocrinology Diabetes mellitus type 1 urology Sexual dysfunction cavernous stz-induced diabetic 2013-08-30 02:07:09 Figure https://plos.figshare.com/articles/figure/_ADSCs_VEGF_increased_cavernous_smooth_muscle_content_in_STZ_induced_diabetic_rats_/785600 <p>Masson’s trichrome staining (A) and semi-quantitative evaluation (B) of smooth muscle/collagen ratio in the cavernous tissue showed this ratio was lower in PBS-treated diabetic rats than age-matched normal rats and was improved by ADSCs or lentivirus-VEGF or ADSCs-VEGF treatment. Scale bar = 50 µm; *<i>p</i><0.05, **<i>p</i><0.01. C: Immunofluorescent staining of cavernous tissue was performed with α-actin and smoothelin in age-matched control rats and STZ-induced DED rats treated with PBS or ADSCs or lentivirus-VEGF or ADSCs-VEGF. Scale bar = 50 µm. D: Representative Western blot for a-actin and smoothelin in normal rats and STZ-induced DED rats after 1 weeks and 4 weeks treatment with PBS, ADSCs, lentivirus-VEGF, or ADSCs-VEGF. E: Quantitative analysis of immunoblotting using Quantity One® software. *<i>p</i><0.05, **<i>p</i><0.01.</p>