Lung infection experiments in <i>K</i>. <i>pneumoniae</i> ATCC 13883 and 10.85 as well as their isogenic porin mutants ΔOpmK35/OmpK36GD.
Alicia Fajardo-Lubián
Nouri L. Ben Zakour
Alex Agyekum
Qin Qi
Jonathan R. Iredell
10.1371/journal.ppat.1007218.g004
https://plos.figshare.com/articles/figure/Lung_infection_experiments_in_i_K_i_i_pneumoniae_i_ATCC_13883_and_10_85_as_well_as_their_isogenic_porin_mutants_OpmK35_OmpK36GD_/7853147
<p>Survival curves after intranasal infection are represented in panels A1 (for ATCC 13883) and A2 (for 10.85). Organ burden after 24, 48 or 72h is represented in panels B1 to D1 for ATCC 13883 and B2 to D2 for 10.85. Violet diamond, ATCC 13883 or 10.85 wild type strains. Pink circle, ΔOmpK35OmpK36GD mutant. For survival studies the results were analysed using Long-rank (Mantel-Cox) test and Gehan-Breslow-Wilcoxon test. To compare bacterial load in organs during lung infection, results were compared using Mann-Whitney unpaired <i>t</i> tests. The analyses were performed using Prism7 (GraphPad Software). The differences between the wild type and the porin mutants were not statistically significant (P < 0.05) in any case.</p>
2019-03-15 17:39:38
member
antibiotic-resistant
adaptation
species
Available sequence data
convergent evolution increases antibiotic resistance
pneumoniae
antibiotic exposure increase
OmpK
pathogen
clone
host
antibiotic resistance
co-regulated partner porin