%0 Figure %A Feng, Hui %A Chen, Ping %A Zhao, Fei %A Nassal, Michael %A Hu, Kanghong %D 2013 %T Impact of individual ε mutations on viral DNA accumulation. %U https://plos.figshare.com/articles/figure/_Impact_of_individual_949_mutations_on_viral_DNA_accumulation_/777056 %R 10.1371/journal.pone.0072798.g003 %2 https://plos.figshare.com/ndownloader/files/1175803 %K Biochemistry %K Nucleic acids %K rna %K microbiology %K Virology %K Viral replication %K Viral nucleic acid %K Viral packaging %K Viral replication complex %K Viral enzymes %K Gastroenterology and hepatology %K Liver diseases %K Infectious hepatitis %K Hepatitis B %K mutations %K viral %K dna %X

HepG2 cells were transfected with the wild-type (wt) HBV expression vector pCH-9/3091, or derivatives containing the mutant 5′ ε sequences shown in Fig. 2B. The + or − signs indicate whether canonical base-pairs could form between residues at the A1-U29 and A2-U28 positions; potential G-U pairs are separately indicated. Viral DNAs from cytoplasmic nucleocapsids were monitored by Southern blotting (top panel) using a 32P-labeled HBV DNA probe; M, 3.2 kb restriction fragment corresponding to a unit length double-stranded linear (dsL) HBV genome. As controls, core protein and β-actin mRNA levels in the source lysates were monitored by Western blotting (middle panel) and RT-PCR (lower panel). Numbers below each lane show the accumulation of viral DNA replicative intermediates, measured by phosphorimaging, relative to those produced by the wild-type HBV construct which was set to 100. Mean values ± standard deviation were derived from two independent experiments.

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