Single-cell transcriptomics identified 22 unique cell types in the human fetal kidney. Mazène Hochane Patrick R. van den Berg Xueying Fan Noémie Bérenger-Currias Esmée Adegeest Monika Bialecka Maaike Nieveen Maarten Menschaart Susana M. Chuva de Sousa Lopes Stefan Semrau 10.1371/journal.pbio.3000152.g001 https://plos.figshare.com/articles/figure/Single-cell_transcriptomics_identified_22_unique_cell_types_in_the_human_fetal_kidney_/7754204 <p>(A) Schematic of kidney epithelium development. (B) Overview of the scRNAseq experiment. (C) Top left: 2D tSNE map of 6,602 human fetal kidney cells. Colors and labels indicate the assigned cell type. (Other panels) tSNE maps indicating expression of <i>SIX2</i>, <i>LHX1</i>, <i>CLDN2</i>, <i>CLIC5</i>, <i>DCN</i>, <i>PDGFRB</i>, and <i>AQP2</i>. Expression is indicated by color; expression values of 1 are plotted in gray. The numerical data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3000152#pbio.3000152.s016" target="_blank">S1 Data</a>. CnT, connecting tubule; DTLH, distal tubule/loop of Henle; End, endothelial cells; ErPrT, early proximal tubule; ICa, interstitial cells a; ICb, interstitial cells b; IPC, interstitial progenitor cell; Leu, leukocyte; Mes, mesangial cell; NPCa, nephron progenitor cells a; NPCb, nephron progenitor cells b; NPCc, nephron progenitor cells c; NPCd, nephron progenitor cells d; Pod, podocyte; Prolif, proliferating cells; PTA, pretubular aggregate; RVCSBa, renal vesicle/comma-shaped body a; RVCSBb, renal vesicle/comma-shaped body b; scRNA seq, single-cell RNA sequencing; SSBm/d, s-shaped body medial/distal; SSBpod, s-shaped body podocyte precursor cells; SSBpr, s-shaped body proximal precursor cells; tSNE, t-distributed stochastic neighbor embedding; tx, transcript; UBCD, ureteric bud/collecting duct; w16, week 16.</p> 2019-02-21 18:50:49 gene expression changes Recent landmark studies gene expression data 22 cell types gene expression dynamics disease-associated marker genes kidney development