Knockdown of Akt2 by RNA interference in gastrocnemius muscle.
Nobuyuki Takenaka
Natsumi Araki
Takaya Satoh
10.1371/journal.pone.0212219.g001
https://plos.figshare.com/articles/figure/Knockdown_of_Akt2_by_RNA_interference_in_gastrocnemius_muscle_/7697720
<p>(a) Two siRNA duplexes against mouse Akt2 (#1 and #2) and a mixture of NC siRNA duplexes were introduced into gastrocnemius muscle fibers of wild-type mice. The expression level of the akt2 mRNA was assessed by quantitative RT-PCR analysis. The expression levels relative to that in NC siRNA-treated muscle are shown as means ± S.E. (n = 6). *<i>P</i> < 0.001. (b) The expression levels of Akt2 and α-tubulin proteins were assessed by immunoblot analysis.</p>
2019-02-08 18:49:54
insulin administration
Akt 2
Rac 1
GTPase Rac 1
protein kinase Akt 2
phosphoinositide 3- kinase
insulin-stimulated Rac 1 activation
Rac 1 acts
Rac 1 activation
glucose transporter GLUT 4
insulin-stimulated GLUT 4 translocation
ectopic expression
guanine nucleotide exchange factor FLJ 00068
RNA
GLUT 4 translocation
Akt 2 expression
glucose uptake