Knockdown of Akt2 by RNA interference in gastrocnemius muscle. Nobuyuki Takenaka Natsumi Araki Takaya Satoh 10.1371/journal.pone.0212219.g001 https://plos.figshare.com/articles/figure/Knockdown_of_Akt2_by_RNA_interference_in_gastrocnemius_muscle_/7697720 <p>(a) Two siRNA duplexes against mouse Akt2 (#1 and #2) and a mixture of NC siRNA duplexes were introduced into gastrocnemius muscle fibers of wild-type mice. The expression level of the akt2 mRNA was assessed by quantitative RT-PCR analysis. The expression levels relative to that in NC siRNA-treated muscle are shown as means ± S.E. (n = 6). *<i>P</i> < 0.001. (b) The expression levels of Akt2 and α-tubulin proteins were assessed by immunoblot analysis.</p> 2019-02-08 18:49:54 insulin administration Akt 2 Rac 1 GTPase Rac 1 protein kinase Akt 2 phosphoinositide 3- kinase insulin-stimulated Rac 1 activation Rac 1 acts Rac 1 activation glucose transporter GLUT 4 insulin-stimulated GLUT 4 translocation ectopic expression guanine nucleotide exchange factor FLJ 00068 RNA GLUT 4 translocation Akt 2 expression glucose uptake