Qin, Chao Zhang, Rui Lang, Yue Shao, Anwen Xu, Aotian Feng, Wenhai Han, Jun Wang, Mengdong He, Wanwei Yu, Cuilian Tang, Jun Bclaf1 interacts with ISGF3 mainly through STAT2. <p>(A) GST pulldown analysis of the interaction between His-STAT1/STAT2/IRF9 and GST-Bclaf1 F2. (B) IB analysis of immunoprecipitates of HEK293T cells co-transfected with Flag-tagged Bclaf1, Ha-tagged STAT1 or STAT2/IRF9 expression plasmids. (C) IB analysis of immunoprecipitates of HEK293T cells co-transfected with Flag-tagged Bclaf1, Ha-tagged IRF9 or STAT2/STAT1 expression plasmids. (D) IB analysis of STAT1, STAT2, IRF9 and Flag-Bclaf1 in nuclear immunoprecipitates of a HEp-2-Flag-Bclaf1 cell line transfected with si-control or si-STAT2 followed by PBS or human IFNα (500U/mL) treatment for 3h. (E) IB analysis of Bio-ISRE pull-down STAT1, STAT2, IRF9 and Bclaf1.</p> STAT;ISRE;Bclaf 1;Bclaf 1 functions;interferon response;HSV -1. Mechanistically;PRV;transcription factor 1;report Bcl -2;Herpes simplex virus type 1;IFN-stimulated response elements;infection;IFN-stimulated gene factor 3;alphaherpesvirus Pseudorabies virus;gene transcription;ISGF 2019-01-25
    https://plos.figshare.com/articles/figure/Bclaf1_interacts_with_ISGF3_mainly_through_STAT2_/7633193
10.1371/journal.ppat.1007559.g007