Comparison of the <i>S</i>. <i>bovis</i> proteome with other schistosome species. OeyHarald ZakrzewskiMartha GravermannKerstin YoungNeil D. K. KorhonenPasi N. GobertGeoffrey NawaratnaSujeevi HasanShihab MartínezDavid M. YouHong LavinMartin K. JonesMalcolm RaganMark A. StoyeJens OleagaAna M. EmeryAidan L. WebsterBonnie RollinsonDavid B. GasserRobin P. McManusDonald KrauseLutz 2019 <p>A) <i>S</i>. <i>bovis</i> centred Venn diagram. The outer ring represents the 11,631 proteins predicted from the <i>S</i>. <i>bovis</i> genome, while the inner 3-way Venn diagram shows the subset of predicted <i>S</i>. <i>bovis</i> proteins that were present in the genomes of <i>S</i>. <i>haematobium</i> (Egypt isolate), <i>S</i>. <i>mansoni and S</i>. <i>japonicum</i>, respectively, based on protein sequence homology. The colours of the inner 3-way Venn diagram correspond to the number of proteins in each intersection from low (light grey) to high (dark blue). A <i>Schistosoma</i> core-proteome of 8,105 proteins was identified as those proteins that had sequence homology matches across all four species. 95 predicted proteins were unique for <i>S</i>. <i>bovis</i>. B) Sequence identity of <i>S</i>. <i>bovis</i> proteins and orthologous proteins from other schistosome species. Proteins of <i>S</i>. <i>bovis</i> and <i>S</i>. <i>haematobium</i> were significantly more conserved (p<10<sup>−15</sup>, two-tailed t-test) than the proteomes of <i>S</i>. <i>bovis-S</i>.<i>-mansoni</i> and <i>S</i>. <i>bovis-S</i>. <i>japonicum</i>. C) Sequence conservation of <i>S</i>. <i>bovis</i> tegument proteins and secreted peptides. <i>S</i>. <i>bovis</i> proteins were mapped to the <i>S</i>. <i>haematobium</i> genome sequence using Exonerate. Tegument proteins and secreted peptides showed an average sequence identity of 98.9% and 97.8%, respectively. All tegument proteins and secreted peptides included in this analysis were shared by both species. D) Analysis of the <i>Schistosoma</i> pan-genome. Proteomes of four <i>Schistosoma</i> species were concatenated, clustered into orthologous groups and mapped to each <i>Schistosoma</i> genome using Exonerate. Colours depict the number of proteins in each intersection from low (light orange) to high (dark orange). A pan-genome of 13,297 orthologous groups and a <i>Schistosoma</i> core-genome of 9,851 proteins were identified.</p>