%0 Figure %A Lía Murall, Carmen %A Jackson, Robert %A Zehbe, Ingeborg %A Boulle, Nathalie %A Segondy, Michel %A Alizon, Samuel %D 2019 %T Epithelial cell growth in 3D raft cultures. %U https://plos.figshare.com/articles/figure/Epithelial_cell_growth_in_3D_raft_cultures_/7621640 %R 10.1371/journal.pcbi.1006646.g002 %2 https://plos.figshare.com/ndownloader/files/14156789 %K testing hypotheses %K ecology-inspired model %K pathogen load %K bottom-up processes %K Human papillomaviruses %K epithelial structure %K modeling approach %K cervicovaginal parameter estimates %K infection symptoms %K tissue cell cultures %K HPV %K 3 D tissue culture %K Epithelial stratification shapes infection dynamics Infections %K Chlamydia trachomatis %K estimate parameters %K infection dynamics %K infection strategies %K pathogen burden %X

A) NIKS grown from a single layer over a period of three weeks. Dark pink layer in week 3 consist of cornified cells that accumulate on the surface. B) Immunoflourescence staining: DAPI (blue) is nuclear staining for cell counting and BrdU (pink) is for identifying cells undergoing division; white dots are added to delineate basal lines. C) Data of NIKS growth over time with model fitting. Shading corresponds to 95% prediction interval, assuming the data follows a Poisson distribution.

%I PLOS Computational Biology