10.1371/journal.pcbi.1006646.g002 Carmen Lía Murall Carmen Lía Murall Robert Jackson Robert Jackson Ingeborg Zehbe Ingeborg Zehbe Nathalie Boulle Nathalie Boulle Michel Segondy Michel Segondy Samuel Alizon Samuel Alizon Epithelial cell growth in 3D raft cultures. Public Library of Science 2019 testing hypotheses ecology-inspired model pathogen load bottom-up processes Human papillomaviruses epithelial structure modeling approach cervicovaginal parameter estimates infection symptoms tissue cell cultures HPV 3 D tissue culture Epithelial stratification shapes infection dynamics Infections Chlamydia trachomatis estimate parameters infection dynamics infection strategies pathogen burden 2019-01-23 18:53:32 Figure https://plos.figshare.com/articles/figure/Epithelial_cell_growth_in_3D_raft_cultures_/7621640 <p>A) NIKS grown from a single layer over a period of three weeks. Dark pink layer in week 3 consist of cornified cells that accumulate on the surface. B) Immunoflourescence staining: DAPI (blue) is nuclear staining for cell counting and BrdU (pink) is for identifying cells undergoing division; white dots are added to delineate basal lines. C) Data of NIKS growth over time with model fitting. Shading corresponds to 95% prediction interval, assuming the data follows a Poisson distribution.</p>