Synergistic effects of azole antifungals and the V-ATPase inhibitor bafilomycin B<sub>1</sub> against <i>C</i>. <i>glabrata</i> wild-type strain. Asuka Minematsu Taiga Miyazaki Shintaro Shimamura Hiroshi Nishikawa Hironobu Nakayama Takahiro Takazono Tomomi Saijo Kazuko Yamamoto Yoshifumi Imamura Katsunori Yanagihara Shigeru Kohno Hiroshi Mukae Koichi Izumikawa 10.1371/journal.pone.0210883.g001 https://plos.figshare.com/articles/figure/Synergistic_effects_of_azole_antifungals_and_the_V-ATPase_inhibitor_bafilomycin_B_sub_1_sub_against_i_C_i_i_glabrata_i_wild-type_strain_/7620569 <p>Checkerboard assay was performed using serial 2-fold dilutions of drugs. (A) Data for the combination of fluconazole and bafilomycin B<sub>1</sub>. (B) Data for the combination of voriconazole and bafilomycin B<sub>1</sub>. Plates were incubated at 35°C for 48 h and the optical density at 600 nm (OD<sub>600</sub>) was determined. The graphs are representative of three independent replicate experiments.</p> 2019-01-23 18:38:07 Candida albicans antifungal agents VPH 2 pH homeostasis findings support V-ATPase function vacuolar membranes reconstituted strains plecomacrolide V-ATPase inhibitor bafilomycin B 1 iron limitation Candida glabrata Vacuolar proton-translocating ATPase Vacuolar proton-translocating ATPase antifungal resistance virulence multidrug resistance pathogen Candida glabrata assembly factor glabrata Δ vph 2 mouse model intracellular ion homeostasis azole antifungal agents VPH 2 gene encoding glabrata wild-type strain calcineurin inhibitor FK 506 stress antifungal strategies acidic pH