Synergistic effects of azole antifungals and the V-ATPase inhibitor bafilomycin B<sub>1</sub> against <i>C</i>. <i>glabrata</i> wild-type strain.
Asuka Minematsu
Taiga Miyazaki
Shintaro Shimamura
Hiroshi Nishikawa
Hironobu Nakayama
Takahiro Takazono
Tomomi Saijo
Kazuko Yamamoto
Yoshifumi Imamura
Katsunori Yanagihara
Shigeru Kohno
Hiroshi Mukae
Koichi Izumikawa
10.1371/journal.pone.0210883.g001
https://plos.figshare.com/articles/figure/Synergistic_effects_of_azole_antifungals_and_the_V-ATPase_inhibitor_bafilomycin_B_sub_1_sub_against_i_C_i_i_glabrata_i_wild-type_strain_/7620569
<p>Checkerboard assay was performed using serial 2-fold dilutions of drugs. (A) Data for the combination of fluconazole and bafilomycin B<sub>1</sub>. (B) Data for the combination of voriconazole and bafilomycin B<sub>1</sub>. Plates were incubated at 35°C for 48 h and the optical density at 600 nm (OD<sub>600</sub>) was determined. The graphs are representative of three independent replicate experiments.</p>
2019-01-23 18:38:07
Candida albicans
antifungal agents
VPH 2
pH homeostasis
findings support
V-ATPase function
vacuolar membranes
reconstituted strains
plecomacrolide V-ATPase inhibitor bafilomycin B 1
iron limitation
Candida glabrata Vacuolar proton-translocating ATPase
Vacuolar proton-translocating ATPase
antifungal resistance
virulence
multidrug resistance
pathogen Candida glabrata
assembly factor
glabrata Δ vph 2
mouse model
intracellular ion homeostasis
azole antifungal agents
VPH 2 gene encoding
glabrata wild-type strain
calcineurin inhibitor FK 506
stress
antifungal strategies
acidic pH