Ferreira Silveira, Guilherme Fanini Wowk, Pryscilla Maria Breckenfeld Machado, Anália Nunes Duarte dos Santos, Claudia Bordignon, Juliano Cryopreservation interferes with the allogeneic lymphoproliferative response. <p>(A) Representative histogram of autologous and allogeneic lymphocyte proliferation of one donor (frh-imdDCs (black), crp-imdDCs (blue) and Lyn-stained lymphocytes without DC stimulation (red)). (B) Frh- (circles) and crp-imdDCs (squares) were co-cultured with autologous (negative control) or allogeneic lymphocytes stained with CellTracker™ Green CMFDA in a 1∶10 ratio of imdDCs:lymphocytes. After five days of co-culture lymphocytes proliferation was analyzed by flow cytometry. Data was analyzed by Student’s <i>t</i> test and the values shown are the means ± SDs of six individual donors. ***<i>p</i>≤0.001. frh: freshly obtained, crp: cryopreserved, imdDCs: immature monocyte derived dendritic cells, Lyn: stained lymphocytes without DC stimulation, Aut lyn: autologous lymphocytes, All lyn: allogeneic lymphocytes.</p> immunology;Immune cells;monocytes;t cells;Immune system;cytokines;immunity;Immune activation;inflammation;Innate immunity;Antigen processing and recognition;Immune response;immunomodulation;interferes;allogeneic;lymphoproliferative 2013-07-31
    https://plos.figshare.com/articles/figure/_Cryopreservation_interferes_with_the_allogeneic_lymphoproliferative_response_/760718
10.1371/journal.pone.0071291.g005