Cryopreservation interferes with the allogeneic lymphoproliferative response.
Guilherme Ferreira Silveira
Pryscilla Fanini Wowk
Anália Maria Breckenfeld Machado
Claudia Nunes Duarte dos Santos
Juliano Bordignon
10.1371/journal.pone.0071291.g005
https://plos.figshare.com/articles/figure/_Cryopreservation_interferes_with_the_allogeneic_lymphoproliferative_response_/760718
<p>(A) Representative histogram of autologous and allogeneic lymphocyte proliferation of one donor (frh-imdDCs (black), crp-imdDCs (blue) and Lyn-stained lymphocytes without DC stimulation (red)). (B) Frh- (circles) and crp-imdDCs (squares) were co-cultured with autologous (negative control) or allogeneic lymphocytes stained with CellTracker™ Green CMFDA in a 1∶10 ratio of imdDCs:lymphocytes. After five days of co-culture lymphocytes proliferation was analyzed by flow cytometry. Data was analyzed by Student’s <i>t</i> test and the values shown are the means ± SDs of six individual donors. ***<i>p</i>≤0.001. frh: freshly obtained, crp: cryopreserved, imdDCs: immature monocyte derived dendritic cells, Lyn: stained lymphocytes without DC stimulation, Aut lyn: autologous lymphocytes, All lyn: allogeneic lymphocytes.</p>
2013-07-31 01:45:26
immunology
Immune cells
monocytes
t cells
Immune system
cytokines
immunity
Immune activation
inflammation
Innate immunity
Antigen processing and recognition
Immune response
immunomodulation
interferes
allogeneic
lymphoproliferative