Cryopreservation interferes with the allogeneic lymphoproliferative response. Guilherme Ferreira Silveira Pryscilla Fanini Wowk Anália Maria Breckenfeld Machado Claudia Nunes Duarte dos Santos Juliano Bordignon 10.1371/journal.pone.0071291.g005 https://plos.figshare.com/articles/figure/_Cryopreservation_interferes_with_the_allogeneic_lymphoproliferative_response_/760718 <p>(A) Representative histogram of autologous and allogeneic lymphocyte proliferation of one donor (frh-imdDCs (black), crp-imdDCs (blue) and Lyn-stained lymphocytes without DC stimulation (red)). (B) Frh- (circles) and crp-imdDCs (squares) were co-cultured with autologous (negative control) or allogeneic lymphocytes stained with CellTracker™ Green CMFDA in a 1∶10 ratio of imdDCs:lymphocytes. After five days of co-culture lymphocytes proliferation was analyzed by flow cytometry. Data was analyzed by Student’s <i>t</i> test and the values shown are the means ± SDs of six individual donors. ***<i>p</i>≤0.001. frh: freshly obtained, crp: cryopreserved, imdDCs: immature monocyte derived dendritic cells, Lyn: stained lymphocytes without DC stimulation, Aut lyn: autologous lymphocytes, All lyn: allogeneic lymphocytes.</p> 2013-07-31 01:45:26 immunology Immune cells monocytes t cells Immune system cytokines immunity Immune activation inflammation Innate immunity Antigen processing and recognition Immune response immunomodulation interferes allogeneic lymphoproliferative