%0 Figure %A Douglas, Lois M. %A Konopka, James B. %D 2019 %T Deletion of the phosphatidylserine synthase gene CHO1 rescues copper sensitivity of the sur7Δ mutant. %U https://plos.figshare.com/articles/figure/Deletion_of_the_phosphatidylserine_synthase_gene_i_CHO1_i_rescues_copper_sensitivity_of_the_i_sur7_i_mutant_/7579721 %R 10.1371/journal.pgen.1007911.g008 %2 https://plos.figshare.com/ndownloader/files/14077592 %K Crp 1 copper exporter %K CHO %K 7 Δ cells %K cause membrane damage %K plasma membrane architecture %K phosphatidylserine %K phospholipid %K drs 2 Δ %K SUR %K 7 Δ %K copper sensitivity %K pathogen Candida albicans exhibits %K Plasma membrane architecture %K plasma membrane organization %K Cup 1 metallothionein %K punctate plasma membrane domains %K MCC %K copper-sensitive mutants %X

(A) Dilutions of cells were spotted onto minimal synthetic medium in the absence or presence of 3 mM CuSO4 and incubated for 48 hr at 30°C. (B) The indicated cell types were incubated in water (- Cu) or 10 μM CuSO4 (+ Cu) for 2 hr at 37°C and then stained with SYTOX Green. The bars represent the average of at least 4 independent assays and error bars indicate SD. * indicates P < 0.02 by t test comparison. Strains used were wild type control DIC185, cho1Δ (YLD222-74 and YLD222-75), sur7Δ (YJA11), and sur7Δ cho1Δ (YLD221-5 and YLD221-6).

%I PLOS Genetics