Douglas, Lois M. Konopka, James B. Cell morphology affects plasma membrane permeabilization by copper. <p>Log phase cells were incubated in a solution of 10 μM CuSO<sub>4</sub> for 2 hr at 37°C and then stained with SYTOX Green. (A) A greater percentage of large <i>sur7Δ</i> cells (≥ 7 μm) stained compared to those of normal size. (B) More budded <i>sur7Δ</i> cells stained with SYTOX Green than unbudded cells.(C) Representative photographs of SYTOX Green stained <i>sur7Δ</i> cells with arrows indicating large cells (>7 μm; upper right panel). Some cells of normal size also stained. Budded cells that stained are shown in the lower right panel. DIC images appear on left. The graphs represent averages of four independent experiments performed on different days. Strains included wild type DIC185 and <i>sur7Δ</i> (YJA11).</p> Crp 1 copper exporter;CHO;7 Δ cells;cause membrane damage;plasma membrane architecture;phosphatidylserine;phospholipid;drs 2 Δ;SUR;7 Δ;copper sensitivity;pathogen Candida albicans exhibits;Plasma membrane architecture;plasma membrane organization;Cup 1 metallothionein;punctate plasma membrane domains;MCC;copper-sensitive mutants 2019-01-11
    https://plos.figshare.com/articles/figure/Cell_morphology_affects_plasma_membrane_permeabilization_by_copper_/7579715
10.1371/journal.pgen.1007911.g006