Cell morphology affects plasma membrane permeabilization by copper.
Lois M. Douglas
James B. Konopka
10.1371/journal.pgen.1007911.g006
https://plos.figshare.com/articles/figure/Cell_morphology_affects_plasma_membrane_permeabilization_by_copper_/7579715
<p>Log phase cells were incubated in a solution of 10 μM CuSO<sub>4</sub> for 2 hr at 37°C and then stained with SYTOX Green. (A) A greater percentage of large <i>sur7Δ</i> cells (≥ 7 μm) stained compared to those of normal size. (B) More budded <i>sur7Δ</i> cells stained with SYTOX Green than unbudded cells.(C) Representative photographs of SYTOX Green stained <i>sur7Δ</i> cells with arrows indicating large cells (>7 μm; upper right panel). Some cells of normal size also stained. Budded cells that stained are shown in the lower right panel. DIC images appear on left. The graphs represent averages of four independent experiments performed on different days. Strains included wild type DIC185 and <i>sur7Δ</i> (YJA11).</p>
2019-01-11 18:38:04
Crp 1 copper exporter
CHO
7 Δ cells
cause membrane damage
plasma membrane architecture
phosphatidylserine
phospholipid
drs 2 Δ
SUR
7 Δ
copper sensitivity
pathogen Candida albicans exhibits
Plasma membrane architecture
plasma membrane organization
Cup 1 metallothionein
punctate plasma membrane domains
MCC
copper-sensitive mutants