Douglas, Lois M. Konopka, James B. Copper sensitivity does not correlate with sensitivity to oxidative stress. <p>Dilutions of the indicated cells were spotted onto (A) YPD containing H<sub>2</sub>O<sub>2</sub> or (B) synthetic medium with CuSO<sub>4</sub>. A <i>cat1Δ</i> catalase deletion served as a control as it is highly sensitive to oxidative stress, but grew to wild type levels on CuSO<sub>4</sub> media. Conversely, the known copper-sensitive mutant <i>cup1Δ crp1Δ</i> grew well on H<sub>2</sub>O<sub>2</sub>, but failed to grow on either CuSO<sub>4</sub> concentration. Hog1 pathway strains <i>pbs2Δ</i>, <i>sskΔ 2</i>, and <i>hog1Δ</i> all displayed sensitivity to increasing concentrations of both H<sub>2</sub>O<sub>2</sub> and CuSO<sub>4</sub>, as did <i>pil1Δ lsp1Δ</i>. <i>sur7Δ</i> was inhibited by copper but grew similar to wild type on H<sub>2</sub>O<sub>2</sub> media. Images are representative of two independent experiments performed on different days. Strains used were wild type, DIC185, <i>sur7Δ</i> (YJA11), <i>pil1Δ lsp1Δ</i> (YHXW21-1), <i>ssk2Δ</i> (YLD185-7), <i>pbs2Δ</i> (YLD197-1), <i>hog1Δ</i> (YLD184-3), <i>cat1Δ</i> (MT505-A), and <i>cup1Δ crp1Δ</i> (KC25).</p> Crp 1 copper exporter;CHO;7 Δ cells;cause membrane damage;plasma membrane architecture;phosphatidylserine;phospholipid;drs 2 Δ;SUR;7 Δ;copper sensitivity;pathogen Candida albicans exhibits;Plasma membrane architecture;plasma membrane organization;Cup 1 metallothionein;punctate plasma membrane domains;MCC;copper-sensitive mutants 2019-01-11
    https://plos.figshare.com/articles/figure/Copper_sensitivity_does_not_correlate_with_sensitivity_to_oxidative_stress_/7579706
10.1371/journal.pgen.1007911.g003