Cytokine expression profile in the dLN of <i>PsSP9</i>-immunized mice a-t one and two months after <i>L</i>. <i>tropica</i> plus <i>Ph</i>. <i>sergenti</i> SGH challenge. Elham Gholami Fabiano Oliveira Tahereh Taheri Negar Seyed Safoora Gharibzadeh Nasim Gholami Amir Mizbani Fatemeh Zali Sima Habibzadeh Daniel Omid Bakhadj Claudio Meneses Kambiz Kamyab-Hesari Alireza Sadeghipour Yasaman Taslimi Fatemeh khadir Shaden Kamhawi Mohammad Ali Mazlomi Jesus G. Valenzuela Sima Rafati 10.1371/journal.pntd.0007067.g006 https://plos.figshare.com/articles/figure/Cytokine_expression_profile_in_the_dLN_of_i_PsSP9_i_-immunized_mice_a-t_one_and_two_months_after_i_L_i_i_tropica_i_plus_i_Ph_i_i_sergenti_i_SGH_challenge_/7579667 <p>BALB/c mice (ten mice per group) were immunized in the right ear three times at two-week intervals with <i>Ph</i>. <i>sergenti</i> SGH, plasmid coding for PsSP9, empty plasmid (VR1020) or PBS. Two weeks after the last immunization, mice were challenged with 10<sup>7</sup> metacyclic <i>L</i>. <i>tropica</i> plus <i>Ph</i>. <i>sergenti</i> SGH in the contralateral ear. Data shown are from one representative of two independent experiments. (A) IFN-γ, (B) IL-5 and (C) the ratio of IFN-γ to IL-5 expression in the dLN of BAB/c mice (four to five mice per group) immunized with PsSP9, <i>Ph</i>. <i>sergenti</i> SGH, VR1020 or PBS, at one month post-challenge with <i>L</i>. <i>tropica</i> plus <i>Ph</i>. <i>sergenti</i> SGH. The obtained results were normalized to the expression level of HPRT. These data are presented as fold change relative to the PBS control group. (D) IFN-γ, (E) IL-5 and (F) the ratio of IFN-γ to IL-5 mRNA expression in dLN of BALB/c mice (five mice per group) immunized with PsSP9, <i>Ph</i>. <i>sergenti</i> SGH, VR1020 or PBS, at two months post challenge with <i>L</i>. <i>tropica</i> plus <i>Ph</i>. <i>sergenti</i> SGH. The obtained results were normalized to the expression level of HPRT. These data are presented as fold change relative to the PBS control. The <i>p</i> value is indicated for each vaccinated group compared with the control plasmid group (VR1020).</p> 2019-01-11 18:37:09 sergenti SGH protein PsSP 9 DTH response arthropod saliva components cutaneous leishmaniasis DNA plasmid coding PsSP 9 tropica infection Ph SP 15 family IL vector-borne disease leishmaniasis CL BALB PsSP 9 antibody response Leishmania tropica Background IFN -γ expression saliva-specific antibody response co-administered Leishmania antigens months post infection PpSP 15 family sergenti saliva