Gholami, Elham Oliveira, Fabiano Taheri, Tahereh Seyed, Negar Gharibzadeh, Safoora Gholami, Nasim Mizbani, Amir Zali, Fatemeh Habibzadeh, Sima Bakhadj, Daniel Omid Meneses, Claudio Kamyab-Hesari, Kambiz Sadeghipour, Alireza Taslimi, Yasaman khadir, Fatemeh Kamhawi, Shaden Mazlomi, Mohammad Ali Valenzuela, Jesus G. Rafati, Sima Cytokine profile expression in the dLN of mice immunized with selected plasmids 48 h after <i>Ph</i>. <i>sergenti</i> SGH injection. <p>BALB/c mice (six mice per group) were intradermally injected in the right ear three times at two week intervals with plasmids encoding one of the five <i>Ph</i>. <i>sergenti</i> salivary proteins (10μg), <i>Ph</i>. <i>sergenti</i> salivary gland homogenate (SGH, 0.5 pair), empty plasmid (VR1020, 10μg) or PBS alone in total volume of 10 μl. Two weeks after the last immunization, the contralateral ear was intradermally challenged with 0.5 pairs of <i>Ph</i>. <i>sergenti</i> SGH. Data shown are from one representative of two independent experiments. (A) IFN-γ, (B) IL-5 and (C) the ratio of IFN-γ to IL-5 mRNA expression in the dLN of BALB/c mice immunized with <i>PsSP9</i>, <i>PsSP40</i>, <i>PsSP41</i>, <i>PsSP44</i>, <i>PsSP52</i>, empty plasmid, and PBS 48 h after challenge with <i>Ph</i>. <i>sergenti</i> SGH were analyzed. The obtained results were normalized to the expression level of HPRT. These data are presented as fold change relative to the PBS control. The <i>p</i> value is indicated for each immunized group compared to the control plasmid group (VR1020).</p> sergenti SGH;protein PsSP 9;DTH response;arthropod saliva components;cutaneous leishmaniasis;DNA plasmid coding;PsSP 9;tropica infection;Ph;SP 15 family;IL;vector-borne disease leishmaniasis;CL;BALB;PsSP 9 antibody response;Leishmania tropica Background;IFN -γ expression;saliva-specific antibody response;co-administered Leishmania antigens;months post infection;PpSP 15 family;sergenti saliva 2019-01-11
    https://plos.figshare.com/articles/figure/Cytokine_profile_expression_in_the_dLN_of_mice_immunized_with_selected_plasmids_48_h_after_i_Ph_i_i_sergenti_i_SGH_injection_/7579661
10.1371/journal.pntd.0007067.g004