Histopathological analysis of the skin response to <i>Ph</i>. <i>sergenti</i> salivary proteins. GholamiElham OliveiraFabiano TaheriTahereh SeyedNegar GharibzadehSafoora GholamiNasim MizbaniAmir ZaliFatemeh HabibzadehSima BakhadjDaniel Omid MenesesClaudio Kamyab-HesariKambiz SadeghipourAlireza TaslimiYasaman khadirFatemeh KamhawiShaden MazlomiMohammad Ali ValenzuelaJesus G. RafatiSima 2019 <p>Histological evaluation of representative ears of BALB/c mice immunized with <i>PsSP9</i>, <i>PsSP40</i>, <i>PsSP41</i>, <i>PsSP42</i>, <i>PsSP</i>52, <i>Ph</i>. <i>sergenti</i> SGH, empty plasmid, or PBS. Two weeks after the last immunization, mice were challenged in the opposite ear with <i>Ph</i>. <i>sergenti</i> salivary gland homogenate. Ear sections stained with hematoxylin and eosin (H&E staining). Sections were analyzed by optical microscopy under 100× and 400× (A) or 100× (B) magnification and the number of leukocytes enumerated microscopically. Bars represent the mean ±SD. Data are representative of two independent experiments (3–5 mice per group). The <i>p</i> value is indicated for each immunized group compared with the control plasmid group (VR1020).</p>