Histopathological analysis of the skin response to <i>Ph</i>. <i>sergenti</i> salivary proteins. Elham Gholami Fabiano Oliveira Tahereh Taheri Negar Seyed Safoora Gharibzadeh Nasim Gholami Amir Mizbani Fatemeh Zali Sima Habibzadeh Daniel Omid Bakhadj Claudio Meneses Kambiz Kamyab-Hesari Alireza Sadeghipour Yasaman Taslimi Fatemeh khadir Shaden Kamhawi Mohammad Ali Mazlomi Jesus G. Valenzuela Sima Rafati 10.1371/journal.pntd.0007067.g003 https://plos.figshare.com/articles/figure/Histopathological_analysis_of_the_skin_response_to_i_Ph_i_i_sergenti_i_salivary_proteins_/7579658 <p>Histological evaluation of representative ears of BALB/c mice immunized with <i>PsSP9</i>, <i>PsSP40</i>, <i>PsSP41</i>, <i>PsSP42</i>, <i>PsSP</i>52, <i>Ph</i>. <i>sergenti</i> SGH, empty plasmid, or PBS. Two weeks after the last immunization, mice were challenged in the opposite ear with <i>Ph</i>. <i>sergenti</i> salivary gland homogenate. Ear sections stained with hematoxylin and eosin (H&E staining). Sections were analyzed by optical microscopy under 100× and 400× (A) or 100× (B) magnification and the number of leukocytes enumerated microscopically. Bars represent the mean ±SD. Data are representative of two independent experiments (3–5 mice per group). The <i>p</i> value is indicated for each immunized group compared with the control plasmid group (VR1020).</p> 2019-01-11 18:37:09 sergenti SGH protein PsSP 9 DTH response arthropod saliva components cutaneous leishmaniasis DNA plasmid coding PsSP 9 tropica infection Ph SP 15 family IL vector-borne disease leishmaniasis CL BALB PsSP 9 antibody response Leishmania tropica Background IFN -γ expression saliva-specific antibody response co-administered Leishmania antigens months post infection PpSP 15 family sergenti saliva