Oberacker, Phil Stepper, Peter Bond, Donna M. Höhn, Sven Focken, Jule Meyer, Vivien Schelle, Luca Sugrue, Victoria J. Jeunen, Gert-Jan Moser, Tim Hore, Steven R. von Meyenn, Ferdinand Hipp, Katharina A. Hore, Timothy P. Jurkowski, Tomasz Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation <div><p>Current molecular biology laboratories rely heavily on the purification and manipulation of nucleic acids. Yet, commonly used centrifuge- and column-based protocols require specialised equipment, often use toxic reagents, and are not economically scalable or practical to use in a high-throughput manner. Although it has been known for some time that magnetic beads can provide an elegant answer to these issues, the development of open-source protocols based on beads has been limited. In this article, we provide step-by-step instructions for an easy synthesis of functionalised magnetic beads, and detailed protocols for their use in the high-throughput purification of plasmids, genomic DNA, RNA and total nucleic acid (TNA) from a range of bacterial, animal, plant, environmental and synthetic sources. We also provide a bead-based protocol for bisulfite conversion and size selection of DNA and RNA fragments. Comparison to other methods highlights the capability, versatility, and extreme cost-effectiveness of using magnetic beads. These open-source protocols and the associated webpage (<a href="https://bomb.bio" target="_blank">https://bomb.bio</a>) can serve as a platform for further protocol customisation and community engagement.</p></div> acid;DNA;TNA;beads;RNA;high-throughput;open-source protocols 2019-01-10
    https://plos.figshare.com/articles/dataset/Bio-On-Magnetic-Beads_BOMB_Open_platform_for_high-throughput_nucleic_acid_extraction_and_manipulation/7575245
10.1371/journal.pbio.3000107