10.1371/journal.ppat.1007515.g008
John V. Dzimianski
John V.
Dzimianski
Brianna S. Beldon
Brianna S.
Beldon
Courtney M. Daczkowski
Courtney M.
Daczkowski
Octavia Y. Goodwin
Octavia
Y. Goodwin
Florine E. M. Scholte
Florine
E. M. Scholte
Éric Bergeron
Éric
Bergeron
Scott D. Pegan
Scott D.
Pegan
Second site of FARV vOTU interaction with di-Ub.
Public Library of Science
2019
novel X-ray crystal structures
Crimean-Congo hemorrhagic fever virus
Recent genomic characterization
ISG 15 fluorogenic substrates
Hughes orthonairovirus species
Nairoviridae family
vOTU substrate preferences
DUB
deubiquitinase activity Post-translational modification
gene product 15
nairovirus genomic diversity
tumor domain protease
2019-01-10 19:09:59
Figure
https://plos.figshare.com/articles/figure/Second_site_of_FARV_vOTU_interaction_with_di-Ub_/7575236
<p>(A) Model of FARV vOTU (reddish orange) bound to K63-linked di-Ub (purple; PDB ID 2JF5). FARV vOTU was overlaid with CCHFV vOTU bound to Ub (PDB ID 3PRP; not rendered) based on secondary structure alignment of the vOTUs. The distal Ub was anchored to the bound mono-Ub by aligning the secondary structure in Coot, followed by manual bond rotations within Lys63 of the proximal Ub in PyMol to model a plausible fit with minimal clashes based on the CCHFV vOTU active site and protease surface. The predicted region of FARV vOTU engagement with the proximal Ub is indicated by a black box. (B) Closeup view of the predicted region, with the residues selected for mutation shown as sticks. Activity of the mutants relative to WT is shown for Ub-AMC, Ub-Rh110, K48 di-Ub FRET-TAMRA, and K63 di-Ub FRET-TAMRA (right).</p>