10.1371/journal.ppat.1007515.g003 John V. Dzimianski John V. Dzimianski Brianna S. Beldon Brianna S. Beldon Courtney M. Daczkowski Courtney M. Daczkowski Octavia Y. Goodwin Octavia Y. Goodwin Florine E. M. Scholte Florine E. M. Scholte Éric Bergeron Éric Bergeron Scott D. Pegan Scott D. Pegan vOTU preferences for different di-Ub linkages. Public Library of Science 2019 novel X-ray crystal structures Crimean-Congo hemorrhagic fever virus Recent genomic characterization ISG 15 fluorogenic substrates Hughes orthonairovirus species Nairoviridae family vOTU substrate preferences DUB deubiquitinase activity Post-translational modification gene product 15 nairovirus genomic diversity tumor domain protease 2019-01-10 19:09:59 Figure https://plos.figshare.com/articles/figure/vOTU_preferences_for_different_di-Ub_linkages_/7575221 <p>(A) Activity towards K48, K63, and K11 linked di-Ub FRET-TAMRA substrates. Values shown are the mean ± standard deviation of two independent experiments. (B) Gel cleavage assay of unlabeled di-Ub, visualized by Commassie Blue staining. (C) Summary of currently investigated vOTU di-Ub activity (present study and [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007515#ppat.1007515.ref032" target="_blank">32</a>]).</p>