<i>nhr-62</i> specifically regulates DR-induced longevity.
Bree N. Heestand
Yidong Shen
Wei Liu
Daniel B. Magner
Nadia Storm
Caroline Meharg
Bianca Habermann
Adam Antebi
10.1371/journal.pgen.1003651.g002
https://plos.figshare.com/articles/figure/_nhr_62_specifically_regulates_DR_induced_longevity_/755098
<p>(A) Survivorship curves for wild-type (N2), <i>eat-2(ad465)</i>, <i>nhr-62(tm1818)</i>, <i>eat-2;nhr-62</i>, and <i>eat-2;nhr-62(dhEx627)</i>. Extra chromosomal array <i>dhEx627</i> (carrying a wild-type <i>nhr-62</i>) in <i>eat-2;nhr-62</i> resulted in a significant increase in lifespan compared to both <i>nhr-62(tm1818)</i> and <i>eat-2;nhr-62</i> mutants (p<0.001). (B) Wild-type (N2) worms with <i>dhEx627</i> exhibited a significant increase in lifespan compared to wild-type (p<0.001). See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003651#pgen.1003651.s002" target="_blank">Figure S2</a> for physiologic traits of overexpression. (C,D) Both wild-type (N2) and <i>nhr-62(tm1818)</i> worms fed <i>daf-2</i> RNAi or <i>cco-1</i> RNAi had a significant increase in lifespan when compared to controls (p<0.001). (E) BDR curve for wild-type (N2) and <i>nhr-62(tm1818)</i> worms. The lifespan of wild-type and <i>nhr-62(tm1818)</i> were not different at the three most concentrated food dilutions, but were significantly different at the remaining 7 dilutions (p<0.001). (F) Survivorship curves for wild-type (N2) and <i>nhr-62(tm1818)</i> fed either 6.45×10<sup>8</sup> CFU/ml or 3.23×10<sup>8</sup> CFU/ml. p-values calculated by the log-rank test.</p>
2013-07-25 02:12:22
genetics
gene expression
RNA interference
Animal genetics
Gene function
Genetic screens
Model organisms
Animal models
Caenorhabditis elegans
Population biology
aging
regulates
dr-induced