Enzymatic activity assay in vitro with serial amount of enzyme at 27 °C or 37 °C. Binbin Li Sisi Li Juan Yin Jiang Zhong 10.1371/journal.pone.0069442.g004 https://plos.figshare.com/articles/figure/_Enzymatic_activity_assay_in_vitro_with_serial_amount_of_enzyme_at_27_176_C_or_37_176_C_/754503 <p>0.25-5 μg bacterially expressed <i>Spodoptera</i> His-Su(var) 3-9 (~70 kDa) was added in the reaction, the “5+” indicates the presence of 5 µM Chaetocin in the reactions containing 5 µg recombinant His-Su(var) 3-9. Reaction product was analyzed with Western blot and the bands of trimethylated H3K9 and total H3 (~18 kDa) were shown in the upper and middle panel, respectively. The SDS-PAGE of reaction mixtures was shown at bottom panel.</p> 2013-07-24 07:04:53 assay vitro serial enzyme 27 37