10.1371/journal.pone.0070377.g002 Anna S. Sahlberg Anna S. Sahlberg Marja Ruuska Marja Ruuska Kaisa Granfors Kaisa Granfors Markus A. Penttinen Markus A. Penttinen PKR inhibition effects on HuR expression and regulation in U937 monocytic cells. Public Library of Science 2013 immunology Immune cells monocytes Immune system cytokines Immune response Major histocompatibility complex microbiology Bacterial pathogens Salmonella Host-pathogen interaction Molecular cell biology cytometry flow cytometry Signal transduction Signaling cascades MAPK signaling cascades Protein kinase signaling cascade Stress signaling cascade Cellular stress responses Infectious diseases Bacterial diseases inhibition hur u937 monocytic 2013-07-22 02:56:54 Figure https://plos.figshare.com/articles/figure/_PKR_inhibition_effects_on_HuR_expression_and_regulation_in_U937_monocytic_cells_/750326 <p>Full length HuR (36 kDa) and HuR cleavage products (CP1; 24 kDa and CP2; 8 kDa) in LPS-stimulated (A) and <i>Salmonella enteritidis</i>-infected (B) PKR inhibitor (PKR+)- or negative control for PKR inhibition (PKR-)-treated U937 Mock, B27g, H9F and E45M cells at 5 h time point. Expression of HuR was determined by SDS-PAGE and Western blotting. HSPA8 was used as a loading control. Results from densitometric analysis of five independent experiments are shown in diagram. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070377#s2" target="_blank">Materials and Methods</a> for details. * = P-value ≤0.06 for HuR, <sup>‡</sup> = P-value ≤0.06 for CP1 and <sup>†</sup> = P-value ≤0.06 for CP2 versus untreated sample at the respective cell line.</p>