10.1371/journal.pone.0070377.g002
Anna S. Sahlberg
Anna
S. Sahlberg
Marja Ruuska
Marja
Ruuska
Kaisa Granfors
Kaisa
Granfors
Markus A. Penttinen
Markus
A. Penttinen
PKR inhibition effects on HuR expression and regulation in U937 monocytic cells.
Public Library of Science
2013
immunology
Immune cells
monocytes
Immune system
cytokines
Immune response
Major histocompatibility complex
microbiology
Bacterial pathogens
Salmonella
Host-pathogen interaction
Molecular cell biology
cytometry
flow cytometry
Signal transduction
Signaling cascades
MAPK signaling cascades
Protein kinase signaling cascade
Stress signaling cascade
Cellular stress responses
Infectious diseases
Bacterial diseases
inhibition
hur
u937
monocytic
2013-07-22 02:56:54
Figure
https://plos.figshare.com/articles/figure/_PKR_inhibition_effects_on_HuR_expression_and_regulation_in_U937_monocytic_cells_/750326
<p>Full length HuR (36 kDa) and HuR cleavage products (CP1; 24 kDa and CP2; 8 kDa) in LPS-stimulated (A) and <i>Salmonella enteritidis</i>-infected (B) PKR inhibitor (PKR+)- or negative control for PKR inhibition (PKR-)-treated U937 Mock, B27g, H9F and E45M cells at 5 h time point. Expression of HuR was determined by SDS-PAGE and Western blotting. HSPA8 was used as a loading control. Results from densitometric analysis of five independent experiments are shown in diagram. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070377#s2" target="_blank">Materials and Methods</a> for details. * = P-value ≤0.06 for HuR, <sup>‡</sup> = P-value ≤0.06 for CP1 and <sup>†</sup> = P-value ≤0.06 for CP2 versus untreated sample at the respective cell line.</p>