Zhang, Huiying Niu, Xiangli Liu, Jia Xiao, Fangming Cao, Shuqing Liu, Yongsheng The H<sup>+</sup> efflux in <i>OsVHA</i><i>-</i><i>A</i>-RNAi lines. <p>(A) Plasma membrane H<sup>+</sup>-ATPase activity was determined in wild type (WT) and three <i>OsVHA-A</i> RNA interference lines (<i>OsV-5</i>, <i>OsV-11</i>, and <i>OsV-18</i>). (B) Plasma membrane H<sup>+</sup>-ATPase proton-pumping measured by the quenching of ACMA fluorescence. Ten micrograms of tonoplast vesicles were applied to detect fluorescence density. (C) The H<sup>+</sup> fluxes determined by micro-test system in wild type and transgenic line (<i>OsV-5</i>) under normal (CK) and 20% PEG6000 treatment (PEG). The positive values mean ion effluxes, whereas negative values show ion influx. Values are means ± SE (n  = 6). Asterisks (*) indicate significant differences from WT at <i>P</i><0.05.</p> crops;cereals;rice;developmental biology;Plant growth and development;ecology;Plant ecology;Plant-environment interactions;genetics;Gene function;Model organisms;Plant and algal models;Molecular cell biology;Signal transduction;Signaling cascades;Stress signaling cascade;Cellular stress responses;Plant science;efflux 2013-07-22
    https://plos.figshare.com/articles/figure/_The_H_efflux_in_OsVHA_A_RNAi_lines_/750309
10.1371/journal.pone.0069046.g005