V-ATPase, PPase activity assays and vacuolar pH measurements in <i>OsVHA</i><i>-</i><i>A</i> RNAi transgenic lines.
Huiying Zhang
Xiangli Niu
Jia Liu
Fangming Xiao
Shuqing Cao
Yongsheng Liu
10.1371/journal.pone.0069046.g004
https://plos.figshare.com/articles/figure/_V_ATPase_PPase_activity_assays_and_vacuolar_pH_measurements_in_OsVHA_A_RNAi_transgenic_lines_/750308
<p>(A) Vacuolar H<sup>+</sup>-ATPase activity and (B) V-PPase activity were determined in wild type (WT) and three <i>OsVHA-A</i> RNA interference lines (<i>OsV-5</i>, <i>OsV-11</i>, and <i>OsV-18</i>). (C) The images showing emission intensities of vacuoles from epidermal root cells loaded with BCECF AM. Results shown are representative. Scale bars = 100 μM. (D) The vacuolar pH values calculated from (C). (E) V-ATPase proton-pumping measured by the quenching of ACMA fluorescence. Ten micrograms of tonoplast vesicles were applied to detect fluorescence density. Each bar represents three replications. Asterisks (*) indicate significant differences from WT at <i>P</i><0.05.</p>
2013-07-22 02:52:36
crops
cereals
rice
developmental biology
Plant growth and development
ecology
Plant ecology
Plant-environment interactions
genetics
Gene function
Model organisms
Plant and algal models
Molecular cell biology
Signal transduction
Signaling cascades
Stress signaling cascade
Cellular stress responses
Plant science
PPase
assays
vacuolar
ph
rnai
transgenic