V-ATPase, PPase activity assays and vacuolar pH measurements in <i>OsVHA</i><i>-</i><i>A</i> RNAi transgenic lines. Huiying Zhang Xiangli Niu Jia Liu Fangming Xiao Shuqing Cao Yongsheng Liu 10.1371/journal.pone.0069046.g004 https://plos.figshare.com/articles/figure/_V_ATPase_PPase_activity_assays_and_vacuolar_pH_measurements_in_OsVHA_A_RNAi_transgenic_lines_/750308 <p>(A) Vacuolar H<sup>+</sup>-ATPase activity and (B) V-PPase activity were determined in wild type (WT) and three <i>OsVHA-A</i> RNA interference lines (<i>OsV-5</i>, <i>OsV-11</i>, and <i>OsV-18</i>). (C) The images showing emission intensities of vacuoles from epidermal root cells loaded with BCECF AM. Results shown are representative. Scale bars  = 100 μM. (D) The vacuolar pH values calculated from (C). (E) V-ATPase proton-pumping measured by the quenching of ACMA fluorescence. Ten micrograms of tonoplast vesicles were applied to detect fluorescence density. Each bar represents three replications. Asterisks (*) indicate significant differences from WT at <i>P</i><0.05.</p> 2013-07-22 02:52:36 crops cereals rice developmental biology Plant growth and development ecology Plant ecology Plant-environment interactions genetics Gene function Model organisms Plant and algal models Molecular cell biology Signal transduction Signaling cascades Stress signaling cascade Cellular stress responses Plant science PPase assays vacuolar ph rnai transgenic