10.1371/journal.pone.0069335.g004 Aymeric Leray Aymeric Leray Sergi Padilla-Parra Sergi Padilla-Parra Julien Roul Julien Roul Laurent Héliot Laurent Héliot Marc Tramier Marc Tramier Comparison of the performance of the non-fitting strategies as a function of the total number of photons for three distinct fractions of interacting donor <i>f<sub>D</sub></i>: 0.25 (A), 0.5 (B) and 0.75 (C). Public Library of Science 2013 Biochemistry proteins Protein interactions biotechnology Bioengineering Biomedical Engineering Molecular cell biology signal processing Image processing biophysics Condensed-matter physics optics non-fitting strategies photons fractions interacting 2013-07-18 03:45:15 Figure https://plos.figshare.com/articles/figure/_Comparison_of_the_performance_of_the_non_fitting_strategies_as_a_function_of_the_total_number_of_photons_for_three_distinct_fractions_of_interacting_donor_f_D_0_25_A_0_5_B_and_0_75_C_/748291 <p>The polar approach is indicated in black, the moments method in blue and the <i>mf<sub>D</sub></i> in red. For all methods, we have reported the estimated <i>f<sub>D</sub></i> and the estimated <i>mf<sub>D</sub></i> value in the left part of the figure. The estimated donor lifetime in presence of the acceptor <i>τ<sub>F,</sub></i> and the estimated mean lifetime <<i>τ</i>> are reported in the right part. In all cases, medians are indicated with markers and error bars correspond to the interquartile ranges of 4096 simulated histograms whose parameters are: <i>τ<sub>F</sub></i> = 1.5 ns, <i>τ<sub>D</sub></i> = 2.5 ns and <i>N<sub>ch</sub></i> = 64 channels (TCSPC simulations) and the simulated values are indicated in dotted lines.</p>