10.1371/journal.pone.0206934.g001 Robin Babadjouni Robin Babadjouni Arati Patel Arati Patel Qinghai Liu Qinghai Liu Kristina Shkirkova Kristina Shkirkova Krista Lamorie-Foote Krista Lamorie-Foote Michelle Connor Michelle Connor Drew M. Hodis Drew M. Hodis Hank Cheng Hank Cheng Constantinos Sioutas Constantinos Sioutas Todd E. Morgan Todd E. Morgan Caleb E. Finch Caleb E. Finch William J. Mack William J. Mack IBA-1 and GFAP staining for reactive glial cells in the medial corpus callosum of mice exposed to filtered air or nanoparticulate matter (nPM). Public Library of Science 2018 air pollution PM-induced neuroinflammation Nanoparticulate matter exposure results complement C 5 deposition complement component C 5a receptor 1 microglial activation C 57BL males corpus callosum nPM Los Angeles region serum C 5 levels matter C 5 deposition C 5 upregulation 2018-11-05 18:48:03 Figure https://plos.figshare.com/articles/figure/IBA-1_and_GFAP_staining_for_reactive_glial_cells_in_the_medial_corpus_callosum_of_mice_exposed_to_filtered_air_or_nanoparticulate_matter_nPM_/7299587 <p>(A) IBA-1 positive cell counts in each experimental group. The nPM group showed significantly greater microglial cell count (n = 8) compared to the filtered air group (n = 8, p = 0.047). * signifies p < 0.05. (B) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for IBA-1 in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. (C) IBA-1 cell body to dendritic process ratio in each experimental group. The nPM cohort had a significantly increased ratio (n = 8) compared to the filter group (n = 8, p < 0.001). ** signifies p < 0.001. (D) GFAP positive cell counts in each experimental group. There was no significant difference in astrocyte cell count between groups (p = 0.983) (E) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for GFAP in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. Error bars represent standard deviation. Scale bars are presented on the lower right corner of the images.</p>