10.1371/journal.pone.0206934.g001
Robin Babadjouni
Robin
Babadjouni
Arati Patel
Arati
Patel
Qinghai Liu
Qinghai
Liu
Kristina Shkirkova
Kristina
Shkirkova
Krista Lamorie-Foote
Krista
Lamorie-Foote
Michelle Connor
Michelle
Connor
Drew M. Hodis
Drew M.
Hodis
Hank Cheng
Hank
Cheng
Constantinos Sioutas
Constantinos
Sioutas
Todd E. Morgan
Todd
E. Morgan
Caleb E. Finch
Caleb
E. Finch
William J. Mack
William
J. Mack
IBA-1 and GFAP staining for reactive glial cells in the medial corpus callosum of mice exposed to filtered air or nanoparticulate matter (nPM).
Public Library of Science
2018
air pollution PM-induced neuroinflammation
Nanoparticulate matter exposure results
complement C 5 deposition
complement component C 5a receptor 1
microglial activation
C 57BL males
corpus callosum
nPM
Los Angeles region
serum C 5 levels
matter C 5 deposition
C 5 upregulation
2018-11-05 18:48:03
Figure
https://plos.figshare.com/articles/figure/IBA-1_and_GFAP_staining_for_reactive_glial_cells_in_the_medial_corpus_callosum_of_mice_exposed_to_filtered_air_or_nanoparticulate_matter_nPM_/7299587
<p>(A) IBA-1 positive cell counts in each experimental group. The nPM group showed significantly greater microglial cell count (n = 8) compared to the filtered air group (n = 8, p = 0.047). * signifies p < 0.05. (B) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for IBA-1 in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. (C) IBA-1 cell body to dendritic process ratio in each experimental group. The nPM cohort had a significantly increased ratio (n = 8) compared to the filter group (n = 8, p < 0.001). ** signifies p < 0.001. (D) GFAP positive cell counts in each experimental group. There was no significant difference in astrocyte cell count between groups (p = 0.983) (E) Below; low magnification representation of region analyzed. Above; filtered air and nPM exposed mice stained for GFAP in the corpus callosum (40x). The upper left hand corner is a high magnification representation of a single cell. Error bars represent standard deviation. Scale bars are presented on the lower right corner of the images.</p>