Liu, Yao Moura, Elisabete C. C. M. Dörr, Jonas M. Scheidelaar, Stefan Heger, Michal Egmond, Maarten R. Killian, J. Antoinette Mohammadi, Tamimount Breukink, Eefjan LysC treatment (37 ˚C, 10 min) of DDM-MraY and SMA-MraY. <p>The influence of 0.1% DDM on the stability of SMA-MraY in the presence of LysC is shown after 10, 40 and 120 min incubation with the detergent at a final concentration of 0.1%. The lanes with the untreated protein are annotated with (<b>*</b>). M indicates the protein marker and the sizes (in kDa) are shown on the left side of the ladder. Intact MraY displays a molecular weight of about 31 kDa and is indicated by a black arrow.</p> styrene-maleic acid copolymers;Escherichia coli membranes;integral membrane protein;DDM;Bacillus subtilis MraY;detergent-free styrene-maleic acid;SMA-wrapped nanodiscs;detergent-free system;pentapeptide translocase MraY 2018-11-05
    https://plos.figshare.com/articles/figure/LysC_treatment_37_C_10_min_of_DDM-MraY_and_SMA-MraY_/7299086
10.1371/journal.pone.0206692.g009