10.1371/journal.pgen.1007776.g006 Hanh Nguyen Hanh Nguyen Sara Labella Sara Labella Nicola Silva Nicola Silva Verena Jantsch Verena Jantsch Monique Zetka Monique Zetka ZHP-4 is required to stabilize RMH-1 at early and late recombination intermediates. Public Library of Science 2018 elegans protein ZHP -4 JM zhp -4 mutants RNF 212-like C paralog ZHP -3 ZHP -4 formation transition elegans ZHP -4 chiasmata COSA RMH CO segregation pro-crossover factors SC MSH DNA hypomorphic mutants exhibit RING domain mutants NCO ZHP -4 function ZHP -4 acts 2018-10-31 17:42:38 Figure https://plos.figshare.com/articles/figure/ZHP-4_is_required_to_stabilize_RMH-1_at_early_and_late_recombination_intermediates_/7279262 <p>GFP::RMH-1 was quantified in the germline nuclei of animals of the indicated genotypes and the numbers binned into six equal zones encompassing the pachytene region: early (A), early-mid (B), mid (C), mid-late (D), late pachytene (E) and pachytene exit (F). All nuclear foci were quantitated. In wild types, RMH-1 appearance and kinetics are similar to a previous report [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007776#pgen.1007776.ref012" target="_blank">12</a>]. Three gonads were scored for each genotype and the data are represented as individual points grouped by genotype and plotted on each Y axis; black bars represent the median +/- interquartile range and Kruskal-Wallis tests and multiple pairwise comparisons were used to assess significant differences among mutants versus wild types (ns = not significant, ** <i>p</i><0.01, *** <i>p</i><0.001). (G,H) Immunolocalization of GFP::RMH-1 (green) and HTP-3 (red) in late pachytene nuclei of the indicated genotypes detects a few small foci in <i>zhp-4(vv103)</i> mutants and multiple brighter foci in <i>zhp-4(vv96)</i> and <i>zhp-4(H26A)</i> mutant nuclei. At pachytene exit, no late RMH-1 foci were detectable in <i>zhp-4(vv103)</i> and <i>zhp-4(vv96)</i> mutants, while 2–4 foci with sizes similar to wild-type foci could be detected on HTP-3-marked tracks in the RING mutant. Scale bars 5 μm.</p>