10.1371/journal.pgen.1007776.g006
Hanh Nguyen
Hanh
Nguyen
Sara Labella
Sara
Labella
Nicola Silva
Nicola
Silva
Verena Jantsch
Verena
Jantsch
Monique Zetka
Monique
Zetka
ZHP-4 is required to stabilize RMH-1 at early and late recombination intermediates.
Public Library of Science
2018
elegans protein ZHP -4
JM
zhp -4 mutants
RNF 212-like C
paralog ZHP -3
ZHP -4
formation
transition
elegans ZHP -4
chiasmata
COSA
RMH
CO
segregation
pro-crossover factors
SC
MSH
DNA
hypomorphic mutants exhibit
RING domain mutants
NCO
ZHP -4 function
ZHP -4 acts
2018-10-31 17:42:38
Figure
https://plos.figshare.com/articles/figure/ZHP-4_is_required_to_stabilize_RMH-1_at_early_and_late_recombination_intermediates_/7279262
<p>GFP::RMH-1 was quantified in the germline nuclei of animals of the indicated genotypes and the numbers binned into six equal zones encompassing the pachytene region: early (A), early-mid (B), mid (C), mid-late (D), late pachytene (E) and pachytene exit (F). All nuclear foci were quantitated. In wild types, RMH-1 appearance and kinetics are similar to a previous report [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007776#pgen.1007776.ref012" target="_blank">12</a>]. Three gonads were scored for each genotype and the data are represented as individual points grouped by genotype and plotted on each Y axis; black bars represent the median +/- interquartile range and Kruskal-Wallis tests and multiple pairwise comparisons were used to assess significant differences among mutants versus wild types (ns = not significant, ** <i>p</i><0.01, *** <i>p</i><0.001). (G,H) Immunolocalization of GFP::RMH-1 (green) and HTP-3 (red) in late pachytene nuclei of the indicated genotypes detects a few small foci in <i>zhp-4(vv103)</i> mutants and multiple brighter foci in <i>zhp-4(vv96)</i> and <i>zhp-4(H26A)</i> mutant nuclei. At pachytene exit, no late RMH-1 foci were detectable in <i>zhp-4(vv103)</i> and <i>zhp-4(vv96)</i> mutants, while 2–4 foci with sizes similar to wild-type foci could be detected on HTP-3-marked tracks in the RING mutant. Scale bars 5 μm.</p>