Expression of a <i>Yersinia pseudotuberculosis</i> Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator Erwan Gueguen Eric Durand Xiang Y. Zhang Quentin d’Amalric Laure Journet Eric Cascales 10.1371/journal.pone.0066615 https://plos.figshare.com/articles/dataset/Expression_of_a_Yersinia_pseudotuberculosis_Type_VI_Secretion_System_Is_Responsive_to_Envelope_Stresses_through_the_OmpR_Transcriptional_Activator/725314 <div><p>The Type VI secretion system (T6SS) is a macromolecular complex widespread in Gram-negative bacteria. Although several T6SS are required for virulence towards host models, most are necessary to eliminate competitor bacteria. Other functions, such as resistance to amoeba predation, biofilm formation or adaptation to environmental conditions have also been reported. This multitude of functions is reflected by the large repertoire of regulatory mechanisms shown to control T6SS expression, production or activation. Here, we demonstrate that one T6SS gene cluster encoded within the <i>Yersinia pseudotuberculosis</i> genome, T6SS-4, is regulated by OmpR, the response regulator of the two-component system EnvZ-OmpR. We first identified OmpR in a transposon mutagenesis screen. OmpR does not control the expression of the four other <i>Y. pseudotuberculosis</i> T6SS gene clusters and of an isolated <i>vgrG</i> gene, and responds to osmotic stresses to bind to and activate the T6SS-4 promoter. Finally, we show that T6SS-4 promotes <i>Y. pseudotuberculosis</i> survival in high osmolarity conditions and resistance to deoxycholate.</p></div> 2013-06-19 01:28:34 Biochemistry proteins DNA-binding proteins genetics gene expression DNA transcription Molecular genetics Gene regulation Genetic screens microbiology Bacterial pathogens Gram negative Microbial pathogens vi secretion responsive stresses ompr transcriptional activator