10.1371/journal.pone.0066430.g001
Upasana Sahu
Upasana
Sahu
Himakshi Sidhar
Himakshi
Sidhar
Pankaj S. Ghate
Pankaj
S. Ghate
Gopal M. Advirao
Gopal
M. Advirao
Sathees C. Raghavan
Sathees
C. Raghavan
Ranjit K. Giri
Ranjit
K. Giri
Increased cytotoxicity, and altered cell and nuclear morphology in MPTQ treated neuro 2a neuroblastoma cells.
Public Library of Science
2013
Molecular cell biology
Signal transduction
Signaling cascades
Apoptotic signaling cascade
Signaling in cellular processes
Apoptotic signaling
organic chemistry
Organic compounds
alkaloids
oncology
Cancer treatment
Chemotherapy and drug treatment
Cancers and neoplasms
Endocrine tumors
Neurological tumors
pediatrics
Pediatric oncology
altered
morphology
mptq
treated
neuro
2a
neuroblastoma
2013-06-18 01:19:49
Figure
https://plos.figshare.com/articles/figure/_Increased_cytotoxicity_and_altered_cell_and_nuclear_morphology_in_MPTQ_treated_neuro_2a_neuroblastoma_cells_/724789
<p>A) Live-Dead assay on neuro 2a neuroblastoma cells was performed after 24 hours of 2.5, 5, 10, 20 or 30 µM of MPTQ treatment. Cells treated with equal amount of DMSO and for same duration served as controls. Fluorescent images from four random fields were captured and were displayed with equal pixel intensity. B) Percentage of dead cells was calculated using multi-cell scoring module of MetaMorph software. Data are expressed as mean±standard deviation of three independent experiments. p value displayed for each treatment was calculated by comparing with control using Student’s t-test. p value ≤0.05 is considered significant. C) Comparative morphology of normal and MPTQ treated neuro 2a cells. Bright field images of normal and 30 µM of MPTQ treated neuro 2a cells demonstrate plasma membrane blebbing (yellow arrow), irregular nuclear compartments (red arrow) only in MPTQ treated cells but not in normal cells after 48 hrs of treatment. D) DAPI stained images exhibit condensed and fragmented nuclei (yellow arrow) only in MPTQ treated but not in normal cells, features typical to apoptotic cells.</p>