10.1371/journal.ppat.1003400.g007
Cierra N. Casson
Cierra
N. Casson
Alan M. Copenhaver
Alan
M. Copenhaver
Erin E. Zwack
Erin
E. Zwack
Hieu T. Nguyen
Hieu T.
Nguyen
Till Strowig
Till
Strowig
Bahar Javdan
Bahar
Javdan
William P. Bradley
William
P. Bradley
Thomas C. Fung
Thomas
C. Fung
Richard A. Flavell
Richard
A. Flavell
Igor E. Brodsky
Igor
E. Brodsky
Sunny Shin
Sunny
Shin
IL-1α and IL-1β control bacterial burden and neutrophil recruitment <i>in vivo</i>.
Public Library of Science
2013
immunology
Immune cells
monocytes
immunity
Immune activation
Immune defense
Immunity to infections
Innate immunity
microbiology
Bacterial pathogens
Gram negative
inflammation
Host-pathogen interaction
pathogenesis
bacterial
neutrophil
recruitment
2013-06-06 01:04:14
Figure
https://plos.figshare.com/articles/figure/_IL_1_and_IL_1_control_bacterial_burden_and_neutrophil_recruitment_in_vivo_/713854
<p>(<b>A</b>) 8–12 week old B6 or <i>Il1r1<sup>−/−</sup></i> mice were infected with 1×10<sup>6</sup> Δ<i>flaA L. pneumophila</i> intranasally (IN). Lungs were plated to quantify CFU per gram. Graph shows the mean ± SEM of three or four infected mice per group. Dashed line represents the limit of detection. (<b>B</b> and <b>C</b>) B6 or <i>Il1r1<sup>−/−</sup></i> mice were infected with 1×10<sup>6</sup> Δ<i>flaA</i> Lp IN. 24 hours post-infection, bronchoalveolar lavage fluid (BALF) was collected and the percentage of neutrophils in the BALF was quantified by flow cytometry. Percentages are reported as the frequency of live cells in the BALF. (B) Representative flow cytometry plots showing the percentage of Gr-1<sup>+</sup>Ly6G<sup>+</sup> neutrophils. (C) Graph showing the percentage of neutrophils. Each point represents an individual mouse and lines indicate the mean of 4 mice per group. (<b>D, E</b>, and <b>F</b>) B6 mice were injected intraperitoneally (IP) with either PBS, 100 µg isotype control antibody (iso), 100 µg anti-IL-1α antibody, 100 µg anti-IL-1β antibody, or 100 µg each of anti-IL-1α and anti-IL-1β (anti-IL-1α/β) 16 hours before infection. The mice were then intranasally infected with either 1×10<sup>6</sup> Δ<i>flaA</i> Lp or mock infected with PBS. (D and E) 24 hours post-infection, BALF was collected and flow cytometry was performed to quantify the percentage of neutrophils. (D) Representative flow cytometry plots showing the percentage of Gr-1<sup>+</sup>Ly6G<sup>+</sup> neutrophils. (E) Graph showing the percentage of neutrophils. Each point represents an individual mouse, lines indicate the mean of 8 mice per group, and error bars represent SEM. Shown are the pooled results of two independent experiments. (F) 72 hours post-infection, the lungs were plated to quantify CFU per gram. Each point represents an individual mouse. Line indicates the mean of 4 infected mice per group with error bars representing SEM. *** is p<0.001 by one-way ANOVA with Tukey post-test or unpaired t-test (C). **is p<0.01 and *is p<0.05 by unpaired t-test. NS is not significant.</p>