10.1371/journal.ppat.1003400.g007 Cierra N. Casson Cierra N. Casson Alan M. Copenhaver Alan M. Copenhaver Erin E. Zwack Erin E. Zwack Hieu T. Nguyen Hieu T. Nguyen Till Strowig Till Strowig Bahar Javdan Bahar Javdan William P. Bradley William P. Bradley Thomas C. Fung Thomas C. Fung Richard A. Flavell Richard A. Flavell Igor E. Brodsky Igor E. Brodsky Sunny Shin Sunny Shin IL-1α and IL-1β control bacterial burden and neutrophil recruitment <i>in vivo</i>. Public Library of Science 2013 immunology Immune cells monocytes immunity Immune activation Immune defense Immunity to infections Innate immunity microbiology Bacterial pathogens Gram negative inflammation Host-pathogen interaction pathogenesis bacterial neutrophil recruitment 2013-06-06 01:04:14 Figure https://plos.figshare.com/articles/figure/_IL_1_and_IL_1_control_bacterial_burden_and_neutrophil_recruitment_in_vivo_/713854 <p>(<b>A</b>) 8–12 week old B6 or <i>Il1r1<sup>−/−</sup></i> mice were infected with 1×10<sup>6</sup> Δ<i>flaA L. pneumophila</i> intranasally (IN). Lungs were plated to quantify CFU per gram. Graph shows the mean ± SEM of three or four infected mice per group. Dashed line represents the limit of detection. (<b>B</b> and <b>C</b>) B6 or <i>Il1r1<sup>−/−</sup></i> mice were infected with 1×10<sup>6</sup> Δ<i>flaA</i> Lp IN. 24 hours post-infection, bronchoalveolar lavage fluid (BALF) was collected and the percentage of neutrophils in the BALF was quantified by flow cytometry. Percentages are reported as the frequency of live cells in the BALF. (B) Representative flow cytometry plots showing the percentage of Gr-1<sup>+</sup>Ly6G<sup>+</sup> neutrophils. (C) Graph showing the percentage of neutrophils. Each point represents an individual mouse and lines indicate the mean of 4 mice per group. (<b>D, E</b>, and <b>F</b>) B6 mice were injected intraperitoneally (IP) with either PBS, 100 µg isotype control antibody (iso), 100 µg anti-IL-1α antibody, 100 µg anti-IL-1β antibody, or 100 µg each of anti-IL-1α and anti-IL-1β (anti-IL-1α/β) 16 hours before infection. The mice were then intranasally infected with either 1×10<sup>6</sup> Δ<i>flaA</i> Lp or mock infected with PBS. (D and E) 24 hours post-infection, BALF was collected and flow cytometry was performed to quantify the percentage of neutrophils. (D) Representative flow cytometry plots showing the percentage of Gr-1<sup>+</sup>Ly6G<sup>+</sup> neutrophils. (E) Graph showing the percentage of neutrophils. Each point represents an individual mouse, lines indicate the mean of 8 mice per group, and error bars represent SEM. Shown are the pooled results of two independent experiments. (F) 72 hours post-infection, the lungs were plated to quantify CFU per gram. Each point represents an individual mouse. Line indicates the mean of 4 infected mice per group with error bars representing SEM. *** is p<0.001 by one-way ANOVA with Tukey post-test or unpaired t-test (C). **is p<0.01 and *is p<0.05 by unpaired t-test. NS is not significant.</p>