Deng, Changwang Li, Ying Liang, Shermi Cui, Kairong Salz, Tal Yang, Hui Tang, Zhanyun G. Gallagher, Patrick Qiu, Yi Roeder, Robert Zhao, Keji Bungert, Jörg Huang, Suming USF1 regulates ESC fate and hematopoietic differentiation by modulating bivalent domains of HS/PC-specific genes. <p>(A) Western blotting assay of hSET1A levels in undifferentiated (day 0) and differentiated (day 13) ESCs comparing pcDNA vector transfected control and AUSF1 overexpressing cells. (B) FACS analysis of c-Kit and Tie2 expressing hemogenic endothelium cell population in pcDNA tranfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 6. (C) FACS analysis of CD41 and c-Kit expressing early hematopoietic stem and progenitor population in pcDNA transfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 10. (D) Real-time RT-qPCR analysis of hematopoietic markers and transcription factors, <i>c-Myb</i>, <i>CD150</i>, <i>CD34</i>, <i>Tal1</i>, <i>Runx1</i>, and <i>HoxB4</i> mRNA transcript levels upon hematopoietic differentiation comparing pcDNA transfected control and two AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05. (E) to (H). ChIP analyses of USF1 binding (E), <u>hSET1A</u> recruitment (F), H3K4me3 enrichment (G), and RNAPII loading (H) at the <i>HoxB4</i> promoter in AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05.</p> Biochemistry;genetics;epigenetics;gene expression;Gene function;hematology;hematopoiesis;regulates;esc;hematopoietic;differentiation;modulating;bivalent;domains 2013-06-06
    https://plos.figshare.com/articles/figure/_USF1_regulates_ESC_fate_and_hematopoietic_differentiation_by_modulating_bivalent_domains_of_HS_PC_specific_genes_/713077
10.1371/journal.pgen.1003524.g006