10.1371/journal.pgen.1003524.g006 Changwang Deng Changwang Deng Ying Li Ying Li Shermi Liang Shermi Liang Kairong Cui Kairong Cui Tal Salz Tal Salz Hui Yang Hui Yang Zhanyun Tang Zhanyun Tang Patrick G. Gallagher Patrick G. Gallagher Yi Qiu Yi Qiu Robert Roeder Robert Roeder Keji Zhao Keji Zhao Jörg Bungert Jörg Bungert Suming Huang Suming Huang USF1 regulates ESC fate and hematopoietic differentiation by modulating bivalent domains of HS/PC-specific genes. Public Library of Science 2013 Biochemistry genetics epigenetics gene expression Gene function hematology hematopoiesis regulates esc hematopoietic differentiation modulating bivalent domains 2013-06-06 00:51:17 Figure https://plos.figshare.com/articles/figure/_USF1_regulates_ESC_fate_and_hematopoietic_differentiation_by_modulating_bivalent_domains_of_HS_PC_specific_genes_/713077 <p>(A) Western blotting assay of hSET1A levels in undifferentiated (day 0) and differentiated (day 13) ESCs comparing pcDNA vector transfected control and AUSF1 overexpressing cells. (B) FACS analysis of c-Kit and Tie2 expressing hemogenic endothelium cell population in pcDNA tranfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 6. (C) FACS analysis of CD41 and c-Kit expressing early hematopoietic stem and progenitor population in pcDNA transfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 10. (D) Real-time RT-qPCR analysis of hematopoietic markers and transcription factors, <i>c-Myb</i>, <i>CD150</i>, <i>CD34</i>, <i>Tal1</i>, <i>Runx1</i>, and <i>HoxB4</i> mRNA transcript levels upon hematopoietic differentiation comparing pcDNA transfected control and two AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05. (E) to (H). ChIP analyses of USF1 binding (E), <u>hSET1A</u> recruitment (F), H3K4me3 enrichment (G), and RNAPII loading (H) at the <i>HoxB4</i> promoter in AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05.</p>