10.1371/journal.pgen.1003524.g006
Changwang Deng
Changwang
Deng
Ying Li
Ying
Li
Shermi Liang
Shermi
Liang
Kairong Cui
Kairong
Cui
Tal Salz
Tal
Salz
Hui Yang
Hui
Yang
Zhanyun Tang
Zhanyun
Tang
Patrick G. Gallagher
Patrick
G. Gallagher
Yi Qiu
Yi
Qiu
Robert Roeder
Robert
Roeder
Keji Zhao
Keji
Zhao
Jörg Bungert
Jörg
Bungert
Suming Huang
Suming
Huang
USF1 regulates ESC fate and hematopoietic differentiation by modulating bivalent domains of HS/PC-specific genes.
Public Library of Science
2013
Biochemistry
genetics
epigenetics
gene expression
Gene function
hematology
hematopoiesis
regulates
esc
hematopoietic
differentiation
modulating
bivalent
domains
2013-06-06 00:51:17
Figure
https://plos.figshare.com/articles/figure/_USF1_regulates_ESC_fate_and_hematopoietic_differentiation_by_modulating_bivalent_domains_of_HS_PC_specific_genes_/713077
<p>(A) Western blotting assay of hSET1A levels in undifferentiated (day 0) and differentiated (day 13) ESCs comparing pcDNA vector transfected control and AUSF1 overexpressing cells. (B) FACS analysis of c-Kit and Tie2 expressing hemogenic endothelium cell population in pcDNA tranfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 6. (C) FACS analysis of CD41 and c-Kit expressing early hematopoietic stem and progenitor population in pcDNA transfected control and AUSF1 overexpressing ESCs upon hematopoietic differentiation at day 10. (D) Real-time RT-qPCR analysis of hematopoietic markers and transcription factors, <i>c-Myb</i>, <i>CD150</i>, <i>CD34</i>, <i>Tal1</i>, <i>Runx1</i>, and <i>HoxB4</i> mRNA transcript levels upon hematopoietic differentiation comparing pcDNA transfected control and two AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05. (E) to (H). ChIP analyses of USF1 binding (E), <u>hSET1A</u> recruitment (F), H3K4me3 enrichment (G), and RNAPII loading (H) at the <i>HoxB4</i> promoter in AUSF1 overexpressing ES clones. Data are shown as mean ± SD. * P<0.05.</p>