Blocking TLR4 and RAGE reduces the bone resorption capacity of S100A12-induced osteoclasts. Miwa Nishida Jun Saegusa Shino Tanaka Akio Morinobu 10.1371/journal.pone.0204140.g004 https://plos.figshare.com/articles/figure/Blocking_TLR4_and_RAGE_reduces_the_bone_resorption_capacity_of_S100A12-induced_osteoclasts_/7114028 <p>Osteoclasts were generated on a bone resorption assay plate with or without S100A12 (1,000 ng/ml), and the additional administration of VIPER (30 μM) and/or an anti-RAGE antibody (10 μg/ml). The resorption lacunae were examined by microscopy after 16 days of culture. <b>(A)</b> The percentage of the resorbed area was quantified and expressed as the mean and SD of 3 independent experiments. * = p < 0.05; *** = p < 0.001. <b>(B)</b> Representative images of resorption pit formation (original magnification × 200).</p> 2018-09-20 18:15:09 CTSK osteoclast differentiation CA 2. Blocking RAGE expression ACP TLR tumor necrosis factor ITG S 100 family member low-molecular-weight calcium-binding protein role TNF IL RANKL S 100A administration CALCR S 100A