10.1371/journal.pone.0066177.s001 Sarah A. Jenkins Sarah A. Jenkins Yi Xu Yi Xu Figure S1 - Characterization of <i>Bacillus anthracis</i> Persistence In Vivo Public Library of Science 2013 microbiology Bacterial pathogens Gram positive Host-pathogen interaction Microbial pathogens pathogenesis Model organisms Animal models mouse Infectious diseases Bacterial diseases anthrax Pulmonology Respiratory infections characterization persistence vivo 2013-06-04 00:22:54 Figure https://plos.figshare.com/articles/figure/Characterization_of_Bacillus_anthracis_Persistence_In_Vivo/711374 <p><b>Detection of vegetative bacilli in the lung by immunofluorescence staining.</b> <b>A</b>, Antibodies against BclA and CW7 are specific for spores and vegetative bacilli, respectively. Spores of <i>B. anthracis</i> Sterne strain 7702 were incubated in DMEM, 10% FBS for 0hr (spores) and 3hrs (vegetative bacilli). Bacteria were then spun onto poly-L-lysine coated coverslips and subjected to immunofluorescence staining. Antibodies against BclA and CW7, a cell wall anchored protein of <i>B. anthracis</i>, were rabbit antibodies raised against purified recombinant proteins of BclA and CW7. Secondary antibodies were goat anti-rabbit IgG–Alexa Fluor 633. Spores were pre-labeled with FITC for visualization. Fluorescence from FITC was barely visible after 3hrs of incubation; therefore, vegetative bacilli were visualized by staining their nuclei with DAPI. <b>B</b>, Detection of vegetative bacilli using anti-CW7 antibodies. A representative image of positive CW7 staining of a lung section from a mouse lung harvested at 2 weeks post-inoculation. Lung sections were fixed, sectioned, and stained with rabbit anti-CW7 antibodies and secondary antibodies conjugated to Alexa Fluor 594 (red), Alexa Fluor 488-conjugated phalliodin (green) and DAPI (blue). Scale bar represents 10 µm.</p> <p>(TIF)</p>