Binding specificity assay of epitope sequence in alanine scan.
Sebastian Hoppe
Frank F. Bier
Markus v. Nickisch-Rosenegk
10.1371/journal.pone.0065837.g005
https://plos.figshare.com/articles/figure/_Binding_specificity_assay_of_epitope_sequence_in_alanine_scan_/708765
<p>Box-whisker-plot (nā=ā12) of the eleven residue long consensus peptide sequence TLIKELKRLGI and its derivative TLIKELKRLAI of cj0669 after incubation with antibodies reactive to <i>C. jejuni</i> (green) and <i>H. pylori</i> (blue). Each box represents 50% of the values, while 98% fall within the whiskers. The median is represented by a horizontal line within each box and the small rectangle corresponds to the mean for each sample. For references the rfi values of the positive control, Rabbit IgG, and negative control, MBP, are indicated on the right for both antibody incubations. For the original sequence a mean value of 38000 A.U. can be observed after incubation with antibody to <i>C. jejuni</i>. This dropped to less than 8000 A.U. after alanine substitution. This represents a drop of roughly 80% in overall intensity. In contrast, neither original nor substituted peptide showed any significant mean values after incubation with the anti-<i>H. pylori</i> antibodies indicating specific binding to the peptide by the anti-<i>Campylobacter</i> antibody.</p>
2013-05-29 02:26:05
Biochemistry
proteins
Recombinant proteins
biotechnology
Applied microbiology
Computational biology
Macromolecular structure analysis
protein structure
microarrays
genetics
gene expression
immunology
Immunologic techniques
immunoassays
Antigen processing and recognition
proteomics
Peptide mapping
Infectious diseases
Bacterial diseases
campylobacter
specificity
assay
epitope
alanine