Alanine Scan of consensus sequence of cj0669 linear epitope.
Sebastian Hoppe
Frank F. Bier
Markus v. Nickisch-Rosenegk
10.1371/journal.pone.0065837.g004
https://plos.figshare.com/articles/figure/_Alanine_Scan_of_consensus_sequence_of_cj0669_linear_epitope_/708764
<p>The Box-whisker-plot (nā=ā15) of the eleven residue long consensus peptide sequence of a cj0669 linear epitope after incubation with three different anti-<i>Campylobacter</i> antibodies. Each box represents 50% of the values, while 98% fall within the whiskers. The median is represented by a horizontal line within each box and the small rectangle corresponds to the mean of each sample. The original sequence TLIKELKRLGI shows a mean fluorescence intensity of about 10000 A.U. For most amino acids within this sequence, replacement by alanine has no to little effect. However, if glycine is replaced by alanine the peptide loses most of its binding activity, clearly seen by a drop of mean rfi to less than 1000 A.U. This equals a drop of more than 90% in intensity.</p>
2013-05-29 02:26:04
Biochemistry
proteins
Recombinant proteins
biotechnology
Applied microbiology
Computational biology
Macromolecular structure analysis
protein structure
microarrays
genetics
gene expression
immunology
Immunologic techniques
immunoassays
Antigen processing and recognition
proteomics
Peptide mapping
Infectious diseases
Bacterial diseases
campylobacter
cj0669
linear