10.1371/journal.pone.0064128
David A. Davis
David A.
Davis
Marco Bortolato
Marco
Bortolato
Sean C. Godar
Sean
C. Godar
Thomas K. Sander
Thomas
K. Sander
Nahoko Iwata
Nahoko
Iwata
Payam Pakbin
Payam
Pakbin
Jean C. Shih
Jean C.
Shih
Kiros Berhane
Kiros
Berhane
Rob McConnell
Rob
McConnell
Constantinos Sioutas
Constantinos
Sioutas
Caleb E. Finch
Caleb
E. Finch
Todd E. Morgan
Todd
E. Morgan
Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
Public Library of Science
2013
neuroscience
Developmental neuroscience
neurogenesis
Behavioral neuroscience
toxicology
Neurotoxicology
Environmental chemistry
atmospheric chemistry
Air quality
Environmental engineering
pollution
Mental health
psychiatry
Anxiety disorders
neurology
Developmental and pediatric neurology
Obstetrics and gynecology
pregnancy
Veterinary medicine
Veterinary neurology
nanoparticles
mice
causes
altered
neuronal
differentiation
depression-like
2013-05-29 02:26:00
Dataset
https://plos.figshare.com/articles/dataset/_Prenatal_Exposure_to_Urban_Air_Nanoparticles_in_Mice_Causes_Altered_Neuronal_Differentiation_and_Depression_Like_Responses_/708760
<div><p>Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticulate matter (nPM). In prior studies, we developed a model for adult rodent exposure to re-aerosolized urban nPM which caused inflammatory brain responses with altered neuronal glutamatergic functions. nPMs are collected continuously for one month from a local freeway and stored as an aqueous suspension, prior to re-aerosolization for exposure of mice under controlled dose and duration. This paradigm was used for a pilot study of prenatal nPM impact on neonatal neurons and adult behaviors. Adult C57BL/6J female mice were exposed to re-aerosolized nPM (350 µg/m<sup>3</sup>) or control filtered ambient air for 10 weeks (3×5 hour exposures per week), encompassing gestation and oocyte maturation prior to mating. Prenatal nPM did not alter litter size, pup weight, or postnatal growth. Neonatal cerebral cortex neurons at 24 hours <i>in vitro</i> showed impaired differentiation, with 50% reduction of stage 3 neurons with long neurites and correspondingly more undifferentiated neurons at Stages 0 and 1. Neuron number after 24 hours of culture was not altered by prenatal nPM exposure. Addition of exogenous nPM (2 µg/ml) to the cultures impaired pyramidal neuron Stage 3 differentiation by 60%. Adult males showed increased depression-like responses in the tail-suspension test, but not anxiety-related behaviors. These pilot data suggest that prenatal exposure to nPM can alter neuronal differentiation with gender-specific behavioral sequelae that may be relevant to human prenatal exposure to urban vehicular aerosols.</p></div>