10.1371/journal.pone.0203521.g005
Sinead T. Loughran
Sinead T.
Loughran
Patrick A. Power
Patrick A.
Power
Paula T. Maguire
Paula T.
Maguire
Samantha L. McQuaid
Samantha L.
McQuaid
Paul J. Buchanan
Paul
J. Buchanan
Ingileif Jonsdottir
Ingileif
Jonsdottir
Robert W. Newman
Robert W.
Newman
Ruth Harvey
Ruth
Harvey
Patricia A. Johnson
Patricia A.
Johnson
Influenza HA specifically attenuates innate cytokines in HKSP-treated cells.
Public Library of Science
2018
hemagglutinin-treated monocyte cultures
Th 17 immunity
virus predisposes individuals
IL -17 responses
IFN
IL -23 levels
Importance Influenza virus
Streptococcus pneumonia
IL -12p IL -23
influenza infection
superinfection
IL -17A
2018-09-07 17:53:54
Figure
https://plos.figshare.com/articles/figure/Influenza_HA_specifically_attenuates_innate_cytokines_in_HKSP-treated_cells_/7061975
<p>The levels of (A) IL-6, IL-23, (B) IL-12p70, (C) IL-27, (D) IL-1β, (E) IL-10 and (F) TGF-β secreted by CD14<sup>+</sup> cells following 24 h treatment with HKSP, H1N1 HA (1μg ml<sup>-1</sup> or 3 μg ml<sup>-1</sup>) or H3N2 HA (1μg ml<sup>-1</sup> or 3 μg ml<sup>-1</sup>) alone or in combination with HKSP (or untreated as a control) were established by ELISA. Each column represents normalised mean cytokine levels + SEM of at least 3 experimental repeats of each treatment/donor. Each ELISA represents normalised results from multiple donors: (A) IL-6 (n = 3), IL-23 (n = 4), (B) IL-12p70 (n = 3), (C) IL-27 (n = 6), (D) IL-1β (n = 4), (E) IL-10 (n = 4), and, TGF-β (n = 3). Statistical analyses were performed to compare cytokine levels in cells exposed to HKSP versus cells exposed to H1N1 HA or H3N2 HA (1μg ml<sup>-1</sup> or 3 μg ml<sup>-1</sup>) in combination with heat-inactivated HKSP, by fitting a One-way ANOVA to the data and using a Sidak test to adjust for multiple testing (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).</p>