The levels of BSP and integrins.
Jie Wang
Li Wang
Bing Xia
Chuanhong Yang
Huangwen Lai
Xiaodong Chen
10.1371/journal.pone.0062936.g005
https://plos.figshare.com/articles/figure/_The_levels_of_BSP_and_integrins_/698703
<p>(A) Flow cytometry (FCM) analyses of αvβ3 protein in the 231BO-BSP27 and 23BO-Scrambled cells, with their negative control in front. The αvβ3 level is lower in the 231-BSP27 cells than in the 231BO-Scrambled cells. (B) FCM analyses for EGFP are used to detect the percentage of the pIRES2-EGFP and pIRES2-hBSP-EGFP transfected cells, while FCM analyses for αvβ3 detect the effect of BSP on the αvβ3 expression. The αvβ3 level is decreased in BSP-depleted cells but is restored by transfection with the pIRES2-hBSP-EGFP. (C) Western blots show that the β3 level is decreased in BSP-depleted cells but is restored by BSP re-expression, with GAPDH as the loading control. (D) Comparisons of the αvβ3, β3 and BSP levels in four cell clones. **p<0.001 <i>vs</i> 231BO-Scrambled.</p>
2013-05-07 02:25:03
biophysics
Cell motility
Model organisms
Animal models
mouse
Molecular cell biology
gene expression
RNA interference
Cell growth
Obstetrics and gynecology
breast cancer
oncology
Basic cancer research
metastasis
Cancers and neoplasms
Breast tumors
bsp