The levels of BSP and integrins. Jie Wang Li Wang Bing Xia Chuanhong Yang Huangwen Lai Xiaodong Chen 10.1371/journal.pone.0062936.g005 https://plos.figshare.com/articles/figure/_The_levels_of_BSP_and_integrins_/698703 <p>(A) Flow cytometry (FCM) analyses of αvβ3 protein in the 231BO-BSP27 and 23BO-Scrambled cells, with their negative control in front. The αvβ3 level is lower in the 231-BSP27 cells than in the 231BO-Scrambled cells. (B) FCM analyses for EGFP are used to detect the percentage of the pIRES2-EGFP and pIRES2-hBSP-EGFP transfected cells, while FCM analyses for αvβ3 detect the effect of BSP on the αvβ3 expression. The αvβ3 level is decreased in BSP-depleted cells but is restored by transfection with the pIRES2-hBSP-EGFP. (C) Western blots show that the β3 level is decreased in BSP-depleted cells but is restored by BSP re-expression, with GAPDH as the loading control. (D) Comparisons of the αvβ3, β3 and BSP levels in four cell clones. **p<0.001 <i>vs</i> 231BO-Scrambled.</p> 2013-05-07 02:25:03 biophysics Cell motility Model organisms Animal models mouse Molecular cell biology gene expression RNA interference Cell growth Obstetrics and gynecology breast cancer oncology Basic cancer research metastasis Cancers and neoplasms Breast tumors bsp