<i>G.</i><i>mellonella</i> virulence assay. Chantal Quiblier Kati Seidl Bernd Roschitzki Annelies S. Zinkernagel Brigitte Berger-Bächi Maria M. Senn 10.1371/journal.pone.0063513.g005 https://plos.figshare.com/articles/figure/_G_mellonella_virulence_assay_/697157 <p>(A) Pathogenicity of Newman pEmpty, Newman Δ<i>secDF</i> pEmpty and Newman Δ<i>secDF</i> pSecDF in <i>G. mellonella</i>. Larvae were monitored every 24 hours. PBS was used as negative control. Three independent experiments were pooled and plotted as Kaplan-Meier survival curve, <i>P</i><0.001. (B) Bacterial burden per (live) larvae was measured in triplicates 24, 48 and 72 h post infection (hpi). The symbols triangle, square, diamond and line correspond to replicates I, II, III and the average, Ø, respectively. The inoculum (CFU/larvae) is shown at time point zero.</p> 2013-05-03 01:59:17 genetics Molecular genetics microbiology Bacterial pathogens staphylococci bacteriology Bacterial physiology Medical microbiology Microbial metabolism Microbial physiology Molecular cell biology cell adhesion proteomics Spectrometric identification of proteins virulence