10.1371/journal.ppat.1007233.g007
Hongbo Guo
Hongbo
Guo
Huib Rabouw
Huib
Rabouw
Anne Slomp
Anne
Slomp
Meiling Dai
Meiling
Dai
Floor van der Vegt
Floor
van der Vegt
Jan W. M. van Lent
Jan W. M.
van Lent
Ryan McBride
Ryan
McBride
James C. Paulson
James C.
Paulson
Raoul J. de Groot
Raoul
J. de Groot
Frank J. M. van Kuppeveld
Frank
J. M. van Kuppeveld
Erik de Vries
Erik
de Vries
Cornelis A. M. de Haan
Cornelis
A. M. de Haan
Virus rolling is driven by NA activity.
Public Library of Science
2018
epithelial host cells
vivo IAV particles
endpoint binding assays
receptor-containing surfaces Interactions
equilibrium binding models
biolayer interferometric analysis
mucus layer
Multiple low-affinity HA-SIA interactions
mucus decoy receptors
Quantitative BLI analysis
NA activity
virus
epithelial cell surfaces
2018-08-13 17:39:27
Figure
https://plos.figshare.com/articles/figure/Virus_rolling_is_driven_by_NA_activity_/6962336
<p>(A) Schematic representation of the experimental set-ups. Colours of the sensors correspond with the colours of the lines in graphs B-D. (A). Biotinylated 3’N+O fetuin (3’N+O fetuin bt) is indicated by the large Xs. Origin of the HA and NA proteins of the viruses used is indicated, as well as the absence or presence of OC during the incubation of the sensors with the viruses. The left, middle and right panels correspond with the graphs shown in B,C, and D (A). The capital R indicates the regeneration of the sensors. (B) 75 pM WSN<sub>HAMtSIN</sub> (with low NA activity) was bound in presence of 10 μM OC to two sensors containing biotinylated 3’N+O fetuin (3’N+O fetuin bt) (loaded to maximum level) for 15 minutes reaching ~15% of the maximum binding level. A control sensor was dipped in PBS. (C) The sensors from B were allowed to bind 30 pM PR8<sub>MtSIN</sub>, in the presence or absence of 10 μM OC. (D) Regenerated sensors were allowed to re-bind WSN<sub>HAMtSIN</sub> at a concentration of 100 pM to determine the degree of de-sialylation that occurred in (C).</p>