10.1371/journal.ppat.1007233.g007 Hongbo Guo Hongbo Guo Huib Rabouw Huib Rabouw Anne Slomp Anne Slomp Meiling Dai Meiling Dai Floor van der Vegt Floor van der Vegt Jan W. M. van Lent Jan W. M. van Lent Ryan McBride Ryan McBride James C. Paulson James C. Paulson Raoul J. de Groot Raoul J. de Groot Frank J. M. van Kuppeveld Frank J. M. van Kuppeveld Erik de Vries Erik de Vries Cornelis A. M. de Haan Cornelis A. M. de Haan Virus rolling is driven by NA activity. Public Library of Science 2018 epithelial host cells vivo IAV particles endpoint binding assays receptor-containing surfaces Interactions equilibrium binding models biolayer interferometric analysis mucus layer Multiple low-affinity HA-SIA interactions mucus decoy receptors Quantitative BLI analysis NA activity virus epithelial cell surfaces 2018-08-13 17:39:27 Figure https://plos.figshare.com/articles/figure/Virus_rolling_is_driven_by_NA_activity_/6962336 <p>(A) Schematic representation of the experimental set-ups. Colours of the sensors correspond with the colours of the lines in graphs B-D. (A). Biotinylated 3’N+O fetuin (3’N+O fetuin bt) is indicated by the large Xs. Origin of the HA and NA proteins of the viruses used is indicated, as well as the absence or presence of OC during the incubation of the sensors with the viruses. The left, middle and right panels correspond with the graphs shown in B,C, and D (A). The capital R indicates the regeneration of the sensors. (B) 75 pM WSN<sub>HAMtSIN</sub> (with low NA activity) was bound in presence of 10 μM OC to two sensors containing biotinylated 3’N+O fetuin (3’N+O fetuin bt) (loaded to maximum level) for 15 minutes reaching ~15% of the maximum binding level. A control sensor was dipped in PBS. (C) The sensors from B were allowed to bind 30 pM PR8<sub>MtSIN</sub>, in the presence or absence of 10 μM OC. (D) Regenerated sensors were allowed to re-bind WSN<sub>HAMtSIN</sub> at a concentration of 100 pM to determine the degree of de-sialylation that occurred in (C).</p>